Mostafa Abdolmaleki

5 Questions 19 Answers 0 Followers

Questions related from Mostafa Abdolmaleki

Some questions about tissue Immunohistofluorescence staining?

1- I read sometimes people added blocking solution after primary Ab and after that added secondary Ab, while, other people normally do before primary Ab and prior that added secondary Ab. - My...

17 July 2016 867 4 View

How keep tissue samples after fix in PFA for immunofluorescence?

I want to do ImmunoFluorescence technique. My target tissue are Chicken Duodenum and Newcastle Disease Virus (NDV). My questions: 1- How long (hours) can I keep Duodenum in Paraformaldehyde 4% to...

27 April 2016 3,657 14 View

How detect virus in chicken duodenum by IHC? and how eliminate the background?

Hi, I am working on chicken duodenum and I faced with 2 problems during working on IHC. 1- I faced with high background and for eliminating that, based on articles, I used 0,1% Sudan Black B in...

24 February 2016 1,469 2 View

Can anyone recommend the best way to remove/reduce background Autofluorescence?

I am using chicken duodenum for IHC. But, I found that my tissue has Autofluorescence. I want to know what is the best way to remove/reduce this Autofluorescence?

05 March 2015 4,152 19 View

Can anybody help me to find antibody ChCD107 ?

Hi I already read the article "Identification of new populations of chicken natural killer (NK) cells" written by "Christine A. Jansen". Can anybody help me how can I find ChCD107 ? Which company...

26 February 2015 9,378 4 View