Mitesh Joshi

6 Questions 9 Answers 0 Followers

Questions related from Mitesh Joshi

How to calculate N/P ratio?

I am planning to use a polymer containing branched/linear PEI of 25K with certain size of DNA (E.g. 4Kb). In order to go ahead with experiments I wish to use various N/P ratios. If anyone can help...

12 August 2024 7,302 1 View

How to coat chitosan on the ZnONP surface ?

I want to coat chitosan on the ZnONP surface to make Chitosan coated ZnONPs. I have tried direct in-situ method for the same where Zinc nitrate and KOH is used as precursor and 5mL of 0.1%...

06 April 2020 8,035 2 View

How to synthesize well dispersed ZnONPs ?

In wet chemical precipitation method of synthesis of ZnONPs, particles are seen to be forming with aggregation and due to which stability issues are seen. I use Zinc nitrate and KOH in simple...

17 November 2019 7,847 1 View

In MTT day 2 of treatment with nanopaticles, removal of previous complete media with FBS is necessary or not?

I am performing MTT of ZnONPs on MCF-7 cells. I have come across two different protocols where, on Day 2 treatment with NPs 1. 100ul of NP dilution prepared in incomplete media is added to wells...

03 September 2019 4,106 3 View

Chitosan coated ZnONPs are not disperssing in water, How can I make them soluble?

Im currently working on synthesis of Chitosan coated ZnO nanoparticles. I have tried coating it with various chitosan concentrations and all concentration ranging from 1% to 0.1% are able to coat...

21 August 2018 1,073 3 View

How do I measure Zeta potential at various pH range?

I have my ZnO nanoparticles and I have to check the zeta potential at various different pH range from 2-12. But usual chemistry of ZnONP tells that they might start dissolving below 5.5 pH or so....

04 March 2018 2,409 11 View