I am planning to use a polymer containing branched/linear PEI of 25K with certain size of DNA (E.g. 4Kb). In order to go ahead with experiments I wish to use various N/P ratios. If anyone can help me with calculation for how to caculate the number of Nitrogen and number of Phosphates in my system.
The N:P. ratio refers here to the ratio of fixed nitrogen (i.e. excluding N2) to phosphate. Fixed nitrogen refers to the sum of nitrate (NO3), nitrite (NO2) and ammonium (NH4). It excludes the always plentiful N2.
The critical N : P ratio is calculated as the ratio between [N] in N-limited plants (grown at Popt) and [P] in P-limited plants (grown at Nopt) that exhibit the same growth rate (treatments to combine are shown by black symbols for r2 and r4).Sep 20, 2004
What is your N/P ratio? - Reef2Reef
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Jul 1, 2023 — Which N/P ratio most closely resembles your N/P ratio? · High nitrates/high phosphates (N/P ~ 22:1) · Low nitrates/low phosphates (N/P ~ 117:1).
We calculated the positive to negative ratio (P/N) by dividing the average optical density for each sample (P) by the average of the negative controls run on the same plate (N) as the sample This has been described in more detail elsewhere [7, 16] .
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