I am currently culturing primary breast cancer epithelial cells from human breast cancer patient. It has been about 3 to 4 months and now epithelial cells are growing well and no more fibroblast already. However, how can I make sure that theses epithelial cells are tumor cells? Do I need to isolate tumor cells by using tumor maker antibodies or How long to keep on doing passaging till only tumor cells remain and normal cell will die off? I just need tumor epithelial cells only in the cultured flasks. Thanks.
I just noted that one paper used expression of MUC1, CD24 and CD44 markers for breast cancer epithelial cells. Is this fine or are there any other makers for tumor epithelial cells of human breast?
We use for primary breast cancer culture the Magnetic cancercstem cell separation kit human CD24-/lowCD44+ cancer and after separation of cells we detected CSC with antibodies to Aldehyde Dehydrogenase 1, BMI-1, CD24, CD44, CD133 and CXCR1/IL-8 RA.
Thanks Ivanov for BCSC. Yes, for breast cancer stem cells (BCSC), I noted that CD24-/CD44+ and CD45- are marker for them.
How about just to isolate human breast cancer epithelial cells from culture flask because I used biopsy tissue. I am not sure currently growing epithelial cells are mixture of normal breast epithelial cells together with tumor breast epithelial cells. I just want tumor breast epithelial cells at this stage. What I found in a paper with regard to breast tumor epithelial cells markers were MUC1, CD24 and CD44. Then also cofirm telomerase activity. Are these OK or Any other easier way to isolate and confirm for breast tumor epithelial cells.
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