Michael Caruso

5 Questions 6 Answers 0 Followers

Questions related from Michael Caruso

What is a reliable method to detect neuronal DNA damage in mouse brain?

I would like to know if there are any standardized methods to detect neuronal double-stranded DNA breaks in mouse brain tissue. Could anybody recommend a method or marker for DNA damage and/or...

21 March 2016 2,804 4 View

Does anyone know of any articles describing the effect of chronic stress during adolescence on genome-wide gene transcription/epigenetics?

I am wondering if there are any papers in which changes in gene transcription (e.g., from microarray or RNAseq experiments) or genome-wide epigenetic modifications (e.g., microarray-based DNA...

04 December 2015 9,206 3 View

Are there any kits validated for the isolation of plasma membrane-associated + cytosolic/nuclear proteins from the same samples of rat brain tissue?

Can anyone recommend a kit that will allow me to isolate protein from the cellular plasma membrane as well as the cytosol and nucleus from the same tissue? I am planning to use the protein I have...

27 May 2015 1,967 1 View

What is the mechanism by which glucocorticoids influence the expression of telomerase reverse transcriptase (TERT)?

I have found several papers showing that stress and/or direct exposure of cells to glucocorticoids can either upregulate or downregulate TERT expression in vitro and in vivo (depending on methods,...

11 September 2014 4,148 1 View

Is there differential expression of glucocorticoid receptor splice variants alpha and beta in the forebrain?

Does anybody know if the two isoforms of the glucocorticoid receptor are differentially expressed (either mRNA or protein) in limbic system structures such as the hippocampus or the amygdala? Most...

22 August 2014 7,207 5 View