Hello everyone
I'm using CRISPR technology to edit a mutated gene in human cells. I used 2 gRNAs, which resulted in the deletion of about 300bp between the two guide RNAs. This was confirmed by gel analysis and Sanger sequencing. To confirm my results further, I sent them for ONP sequencing to get the percentage of deleted sequences. I counted the number of deletions and divided it by the total number of reads. Is this correct? I used the alignment and amplicon tools in epi2me, and I did not get any useful results. Any help is appreciated.