Help with prime editing experiment ?

12 August 2023 0 121 Report

Hello, I'm planning to design a pegRNA for my prime editing experiment. I read a few relevant papers but I'm still very confused. Any help is really appreciated. Regards, Melika

More Melika Fard's questions See All

Is it possible to do simulation for laser ablation in water using COMSOL or Lumerical?

Solving heat transfer equation during laser ablation in air is done with comsol and there are some tutorial videos, is it possible to do do simulation for laser heating during ablation in liquid....

15 April 2024 9,515 1 View

How to find the value for MAGMOM?

Hi. I am working on a project with VASP with MoO3 lattice and recently I found out that I have to consider spin polarization in my calculations. So, now I do not know what value I should consider...

24 September 2023 4,559 2 View

Treatment of Stacking Pad in lateral flow test????

Hi everyone! I want to use a cellulose pad as Stacking Pad in my Lateral Flow test. how can i treat this pad??? Thanks for any help!

31 July 2023 2,791 2 View

How could we avoid aggregation of gold nanoparticles during antigen conjugation??

Hi Despite considering the suitable pH of the target antigen for conjugation with the help of NAOH, after adding the target antigen to PH-adjusted gold solution, and after adding 1% BSA, and...

25 July 2023 2,600 2 View

How could we avoid aggregation of gold nanoparticles during antigen conjugation??

Hi Despite considering the suitable pH of the target antigen for conjugation with the help of NAOH, after adding the target antigen to PH-adjusted gold solution, and after adding 1% BSA, and...

25 July 2023 3,779 3 View

How to calculate atom positions in Primitive orthorhombic lattice?

Hi everyone, I need to add an acetone to a catalyst surface with the primitive orthorhombic structure and I need a help to how predict the atom positions manually (without the help of VESTA or...

29 June 2023 1,730 1 View

How to find current topics related to interior architecture ?

Current topic & new topic & hot topic

23 June 2023 6,675 1 View

How can we, as an interior architect, change people's behavior and mood?

Whats your idea about that ...

07 May 2023 2,203 2 View

How can I remove a research from my ResearchGate account?

I wanted to delete a research from my ResearchGate account, but instead of deleting it from ResearchGate, I deleted it from my profile. Now how can I remove from researchgate considering that it...

03 April 2023 1,578 4 View

In GARCH models, both the error term and the variance term are squared, while the errors can be negative, so how can it be combined with ARIMA models?

It is said that in order to combine garch models with arima models, their results are added together, while garch results are positive in every step. Is there anything wrong with this combination?...

19 February 2023 6,643 2 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How to get links for copyrights for papers?

how to get links for copyrights for papers?

06 August 2024 7,410 1 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How do i get an account to upload my published papers?

need to open an account to upload my published papers

01 August 2024 9,255 1 View

How can I select cells in prime editing without fluorescence-activated cell separation?

31 July 2024 8,551 2 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

Any tips on how to increase the chances of getting a response?

I've been using ResearchGate for a while now and have found many useful research papers that are beneficial for my work. However, I've noticed that many authors do not respond to my messages....

31 July 2024 7,771 5 View

I have no added any resarch paper yet but showing three paper? how to delete it?

I have not addede any paper yet but it has selected 3 papers which are not mine in my account. i want to delete that information. please help me

30 July 2024 3,743 0 View

Are you looking for research collaboration ?

we have few papers ready for submission, and we need one co-author for each article who can pay article fee. Interested authors may text here or contact me on my following email id [email protected]

29 July 2024 6,626 0 View