30 Questions 294 Answers 0 Followers
Questions related from Masood Sepehrimanesh
I extracted the total RNA from prostate tissue of rat and convert it to cDNA. Then, I run a PCR with specific F and R primers for p53. I looking for 149 bp band but I found only one band with...
10 October 2015 1,391 7 View
There are several indexing for scientific articles. Which of the following is better? ISI, Pubmed, Scopus, ISC, Scholar and etc.
10 October 2014 10,075 3 View
Article are citedin another article as (First author, et al. Year). However, the contact information mostly presented for corresponding author. Please discuss you idea that which of these position...
10 October 2014 6,893 2 View
In statistical analysis,to find significant differences, you must use post hoc tests such as Tukey, LSD, bonferroni etc. Which of these tests is better?
01 January 2014 5,223 20 View
Impact factor vs. high quality research publication
12 December 2013 4,102 14 View
In data that obtained in a research, you maybe collect some values that differ but p>0.05 (differences), or p
11 November 2013 6,872 35 View
In 2-DE, after electrophoresis, gel scanning and analysis with Melanie, each spot has 2 values: Intensity and Volume. Which of these two values is more important for showing expression differences?
11 November 2013 6,339 2 View
Spot analysis and confirmation of data by other methods have been reported in valuable articles. is it necessary or only is the additive method?
11 November 2013 9,817 3 View
Is it important to publish an article in ISI journals or justly journals indexed in scopus or pubmed are sufficient?
10 October 2013 3,383 2 View
Proteomics or protein related journals
10 October 2013 1,101 4 View
Acrylamide and bisacrylamide are monomers and neurotoxins. They can damage lab personnel. They are used for polyacrylamide gel preparation to separate proteins and low molecular weight acid...
10 October 2013 8,015 5 View
In 2DE, mixtures of proteins are separated by two properties (Mw and pI) in two dimensions. Therefore, each spot must be equal to one identical protein. In mass analysis I found more than one...
09 September 2013 4,087 7 View
Springer, Elsevier, Scince direct, or KARAGER ?
08 August 2013 438 11 View
Hypothesis is the one of the first step in conducting a research. Are you hear about journals that publish hypothesis?
08 August 2013 1,547 13 View
Please discuses your ideas
08 August 2013 8,721 8 View
2DE map of rat testes were prepared and stained by silver nitrate. I also isolated the spots from the gels but one of my friends advised that "you must run a preparative 2DE and stain it by CBB to...
08 August 2013 6,283 4 View
High IF journal with article processing charge or free low IF journal?
07 July 2013 9,663 19 View
Standard error of mean (SEM) and standard deviation (SD) are commonly used in papers to show deviation of data. Which is the best?
07 July 2013 9,424 13 View
Q10 is a measure of the proportional increase in velocity of enzyme, when temperature increase 10 C.
07 July 2013 4,203 1 View
Cell phone induce stress include oxidative stress to cells and tissues.Antioxidant will face off with this dangerous molecules. Everyone that know its mechanism, please indicate the most...
07 July 2013 6,190 3 View
I want to analysis the effects of stress on antioxidant defense systems in a specific organ. Which of the following samples is better: Serum or tissue fluid or tissue itself? Please discuss your...
07 July 2013 8,880 14 View
PAGE apparatus consist of tank, power supply, electrodes, glass slides and .... . Which of the mentioned companies apparatus is the most simple to use? which is conventional? which is cheaper?
07 July 2013 6,392 21 View
Usually, scientists use the antiprotease cocktail to inhibit four common types of proteases.
07 July 2013 2,123 8 View
Is there positive or negative correlation?
07 July 2013 4,566 12 View
Line art is one of the shapes of data presented in scientific papers. Which shape of line art is better: 1. Broken line that attaches several points together (Created by Excel or ...)? 2....
07 July 2013 4,004 5 View
Protein design is the design of new protein molecules, either from scratch or by making calculated variations on a known structure. Therefore, Is it possible to design highly active de novo...
06 June 2013 4,532 2 View
I ran a proteomic research and used 2 dimensional electrophoresis (2DE). After that, I stained the gels with silver staining but the intesity of spot in my gel is low.
06 June 2013 9,253 6 View
Which of these two technique is better to conduct an proteomic research? Gel based 2 DE or gel free LC-MS?
06 June 2013 7,774 5 View
I have an eluent of protein which contain PBS buffer.
06 June 2013 2,282 11 View
I need a trial software to check it and if it is good I will buy it.
06 June 2013 1,905 5 View
