Q10 is a measure of the proportional increase in velocity of enzyme, when temperature increase 10 C.
Make some experiments. Eg. change the pH of reaction solution but keep temperature constant and measure the rate of reaction.
I extracted the total RNA from prostate tissue of rat and convert it to cDNA. Then, I run a PCR with specific F and R primers for p53. I looking for 149 bp band but I found only one band with...
09 October 2015 1,428 7 View
There are several indexing for scientific articles. Which of the following is better? ISI, Pubmed, Scopus, ISC, Scholar and etc.
09 October 2014 10,109 3 View
Article are citedin another article as (First author, et al. Year). However, the contact information mostly presented for corresponding author. Please discuss you idea that which of these position...
09 October 2014 6,957 2 View
In statistical analysis,to find significant differences, you must use post hoc tests such as Tukey, LSD, bonferroni etc. Which of these tests is better?
31 December 2013 5,268 20 View
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11 December 2013 4,141 14 View
In 2-DE, after electrophoresis, gel scanning and analysis with Melanie, each spot has 2 values: Intensity and Volume. Which of these two values is more important for showing expression differences?
10 November 2013 6,395 2 View
Spot analysis and confirmation of data by other methods have been reported in valuable articles. is it necessary or only is the additive method?
10 November 2013 9,856 3 View
In data that obtained in a research, you maybe collect some values that differ but p>0.05 (differences), or p
10 November 2013 6,921 35 View
Is it important to publish an article in ISI journals or justly journals indexed in scopus or pubmed are sufficient?
09 October 2013 3,432 2 View
Proteomics or protein related journals
09 October 2013 1,148 4 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
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I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...
29 July 2024 7,198 9 View
I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.
29 July 2024 6,115 0 View
Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...
27 July 2024 5,748 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...
24 July 2024 6,592 5 View
I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...
23 July 2024 9,938 1 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View