To measure ER stress in Arabidopsis plants grown on 1/2 MS media, you can assess ER stress markers (e.g., BiP or bZIP60 expression) using quantitative PCR or reporter gene assays. Here’s a brief protocol:

For survival/growth rates, measure root lengths or fresh weight before and after treatment. Use replicates (n ≥ 3) for statistical analysis. Let me know if you need further details!

Dear Raghad

Thank you so much for your response; I appreciate it.

My main goal of the experiment is to phenotypically study the impact of different concentrations of ER stress in my mutant n line compared with other negative and positive controls, I need help to find calculations ( volume for the TM) to be added into the media ( 1/2 MS), for example;le if ia m preparing 500 ml media with final concentration of ( 0.04 ug/ml, 0.08ug/ml )

thank you again