Marie Humbert-Claude

12 Questions 23 Answers 0 Followers

Questions related from Marie Humbert-Claude

Why mirtazapine may "loose" its sedative effect at doses above 30mg ?

Mirtazapine acts as an antagonist of alpha2 adrenergic presynaptic receptors, leading to an increase of 5HT and NA. This is the underlying mechanism of its antidepressant effect. But , its also a...

19 April 2018 8,508 4 View

Would you know which is the most powerful prescription software?

Hello ! Some prescription software are not precise for prescription for drugs, specially for injectable drugs. It could even be dangerous sometimes. Do you use and do you recommand a software...

08 April 2018 113 2 View

What mechanism underlies the antidepressant effect of the atypical antiipsychotics?

Some atypical antipsychotics are used in addition to antidepressants to treat episodes of severe depression, at a lower dosage than those that induce an antipsychotic effect. But what mechanism...

15 February 2018 7,697 4 View

What is the best technique to separate neurons astrocytes and microglia from an adlut mouse brain ?

I would like to analyse mRNA and different isoforms of a protein (but not necessary at the same time) in neurons, astrocytes and microglia from a brain of adult mouse. (I have no antibodies to...

21 April 2016 1,443 7 View

We co-transfected HEK cells with plasmid containing CMV and NRE : Is it possible to have a competition between the two promoters?

Hello, I am looking for article references that evaluate interactions between two promoters in a context of co-transfection in HEK cells: It seems that we have interactions : the plasmid...

11 December 2015 8,227 3 View

Is CMV promoter activated by some transcription factors, activated by inflammation?

We are searching for the binding sites of all transcription factor on CMV promotor, because we are wondering if CMV promoter is activated by IFng for instance .

07 September 2015 6,179 4 View

Can HEK293 cells survive during 4 days in DMEM without serum?

Hello. I need to incubate HEK 293 T cells with a molecule that interfere with serum. So, is it possible to make this incubation in DMEM without serum during 4 days?

12 August 2015 3,647 6 View

Can we use sterilizing filtration (0.22 microm) for adeno-associated virus (AAV1) ?

I would like to filter AAV1 with 0.22 micron filter ,after have made iodixanol gradient and microconcentration because I saw some impurities. But I do not know if it is recommended. ( risk of...

05 August 2015 9,219 3 View

Are mice C57Bl6J from the strain RccHSD from Harlan laboratories suitable for a model of Parkinson's disease?

The mice from the strain C57Bl6J OLA HSD from Harlan Laboratories are mutated on the alphasynuclein gene. We have spent lot of time understanding why, after injection of LPS into the nigra, the...

18 April 2015 5,509 5 View

Covid -19 : Does anyone have already tried to block ECA2 ?

Hello everybody! Can someone may have the answers to the following questions ? 1. Is it correct that ACE-2 (Angiotensin Conversion Enzyme - 2) is a functional receptor for SARS-CoV , and probably...

01 January 1970 2,925 15 View

Covid-19: Should we really switch from Renine -Angiotensin inhibitors (-pril or -sartan) to calcium channel inhibitoirs (-dipine) ?

Hello ! Do anybody know a clinical study where number of Covid19 cases has been compared between hypertensive patients taking renine-angiotensine system inhibitors with the one that are under...

01 January 1970 5,417 7 View

Covid-19: Should we avoid NSAIDs ?

Hello ! Does anyone know of the study that shows that NSAIDs increase the expression of ACE2? Do anyone know is there is a clinical study which shows that NSAIDs really worsens the symptoms of...

01 January 1970 9,261 3 View