In my lab , very often the distribution of neutrophils is not uniform and over 80% are present in the tail . I want to know how to prevent this tailing .
Emmanuel Ifeanyi Obeagu
If the edge of the spreader is not smooth, ragged tail of the smear is formed. This is known as tailing of the smear. Such a smear shows irregular distribution of the leucocytes (with majority of neutrophils at the tail end and reduced number of neutrophils in the rest of the smear) resulting in a fallacious DLC. The spreader slide should have a smooth end to prevent the tail end of the smear from being irregular.
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Khalid Al-Salhie
See this link
https://www.slideshare.net/mobile/bahoran03/peripheral-blood-smear-examination
Regards
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