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Questions related from Liu-qing Chen
Hello! I'm new in cell biology research. i have question about methodology to study cell growth. When comparing control cells with gene knockout cells, I found that the gene knockdown...
02 March 2024 3,497 3 View
Hello everyone! I have tried use the Ig k signal sequence and PDGFR anchor sequence combination to express my target protein in HEK 293. But the target protein located almost all in nuclear...
16 September 2022 1,242 2 View
Hello everyone! I use pCDH-CMV-MCS-EF1-Neo vector to transfect Hek293 cells. During culture of the stalbe cell lines, i found my GOI expression level is high. However, we hope a lower...
11 November 2021 8,860 1 View
I have mutated the catalytic residues (nucleophile or acid/base) of a GH5-29 subfamily enzyme, which naturally use glycosphingolipids as substrate. Unexpectly, the mutants still remaining ~ 60%...
09 September 2019 1,148 0 View
Hi ! Recently, i have did some site directed mutate reaction. However, all the six PCR reaction product have some problem demonstrated by sequencing, the mutated sequence have one inserted...
03 June 2017 4,797 3 View
As we don't have computer cluster, and also not good at linux system, so i want others do abinitio model for me. i have using robetta generated the 3mer and 9mer fragment of my protein, i have...
16 January 2017 6,691 1 View
Hello! Anybody knows how to perform molecular replacement using all protein from the PDB as searching templates? which software can perform this function, or we write scripts ourselves? In...
22 August 2016 9,810 9 View
