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Hello everyone! I have tried use the Ig k signal sequence and PDGFR anchor sequence combination to express my target protein in HEK 293. But the target protein located almost all in nuclear...
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Hello everyone! I use pCDH-CMV-MCS-EF1-Neo vector to transfect Hek293 cells. During culture of the stalbe cell lines, i found my GOI expression level is high. However, we hope a lower...
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Hi ! Recently, i have did some site directed mutate reaction. However, all the six PCR reaction product have some problem demonstrated by sequencing, the mutated sequence have one inserted...
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