Laura Prendergast

11 Questions 2 Answers 0 Followers

Questions related from Laura Prendergast

Does FIV pseudotyped with VGV-G express both gp120 and VGV-G on the surface?

I'm looking for information about pseudo-typing FIV with VSV-G, which improves transduction efficiency. Does FIV pseudo-typed with VSV-G express both the VSV-G and the usual FIV coat proteins?

12 July 2021 490 0 View

How much of the original FIV construct is necessary/useful for engineered lentivector?

I am constructing a lentiviral vector, replacing pretty much all of one of the hetero-diploid constructs with my transgenes. The vector is intended to be replication competent. Other than the...

13 May 2021 9,746 0 View

Can you provide assistance with the design of an FIV-based vector?

I'm designing an FIV-based delivery system. FIV has a heterodiploid RNA genome: I'm intending to use one strand for the FIV's ordinary functions, and replace the other (the cargo construct),...

28 April 2021 7,201 0 View

Does anyone have a sequence for the human CD34 promoter?

I'm looking for the human CD34 promoter sequence to engineer into a vector construct. Can anyone recommend a good source for this sequence? Thanks very much, Laura

26 February 2021 8,865 1 View

Is anyone else having a similar situation?

I'm looking for the genetic sequence for the TAR region of HIV. If I put Transactivator Response Region, and HIV, and "genetic sequence: into a browser...is it not unreasonable to expect to be...

11 February 2021 1,020 5 View

How is an mRNA vaccine scalable for millions of recipients?

I actually have several questions: 1) Where do I go to learn about the physical chemistry of the interaction between ribosomes and RNA made with pseudouridine or 1-methylpseudourine? 2) Are...

02 February 2021 8,081 1 View

Is There Any Documentation of Replication Competent Lentiviruses (RCL)Through Recombination?

It is my understanding that current 3rd and 4th generation gene delivery systems must be tested to ensure they do not give rise to an RCL, before being approved for in vivo use. Has there ever...

03 December 2020 9,229 1 View

What are the Minimal Requirements for Dimerization of the Feline Immunodeficiency Virus Genome?

I'm looking for references on the minimal requirements for dimerization of the FIV genome. From what I understand, the FIV genome is composed of 2 RNA constructs that are dimerized before being...

24 November 2020 604 0 View

Where can I find an IRES sequence?

Where can I find the DNA sequence for an IRES to control expression of GFP in a plasmid intended for expression in T cell lines?

16 May 2015 2,000 3 View

What are standard assays for cytotoxicity? What if your transfected protein is cytotoxic?

I'm planning to transfect T-cells with a fusion protein that is up-regulated in the presence of HIV's tat molecule, which is apparently capable of increasing the expression levels of TAR-labeled...

22 February 2015 9,405 3 View

Is anyone interested in becoming a mentor for assistance/guidance with grant writing for a gene therapy project?

I'm a Molecular Biologist with an MS in Recombinant DNA Technology. I'm trying to get funding for proof-of-concept experimentation to validate the mechanism of an FIV-based vector for delivering...

27 July 2013 3,420 0 View