8 Questions 2 Answers 0 Followers
Questions related from Laura Prendergast
I am constructing a lentiviral vector, replacing pretty much all of one of the hetero-diploid constructs with my transgenes. The vector is intended to be replication competent. Other than the...
13 May 2021 9,698 0 View
I'm designing an FIV-based delivery system. FIV has a heterodiploid RNA genome: I'm intending to use one strand for the FIV's ordinary functions, and replace the other (the cargo construct),...
28 April 2021 7,163 0 View
I'm looking for the human CD34 promoter sequence to engineer into a vector construct. Can anyone recommend a good source for this sequence? Thanks very much, Laura
26 February 2021 8,482 1 View
I'm looking for the genetic sequence for the TAR region of HIV. If I put Transactivator Response Region, and HIV, and "genetic sequence: into a browser...is it not unreasonable to expect to be...
11 February 2021 839 5 View
I actually have several questions: 1) Where do I go to learn about the physical chemistry of the interaction between ribosomes and RNA made with pseudouridine or 1-methylpseudourine? 2) Are...
02 February 2021 8,030 1 View
It is my understanding that current 3rd and 4th generation gene delivery systems must be tested to ensure they do not give rise to an RCL, before being approved for in vivo use. Has there ever...
03 December 2020 9,191 1 View
Where can I find the DNA sequence for an IRES to control expression of GFP in a plasmid intended for expression in T cell lines?
16 May 2015 1,942 3 View
I'm planning to transfect T-cells with a fusion protein that is up-regulated in the presence of HIV's tat molecule, which is apparently capable of increasing the expression levels of TAR-labeled...
22 February 2015 9,352 3 View
