I actually have several questions:
1) Where do I go to learn about the physical chemistry of the interaction between ribosomes and RNA made with pseudouridine or 1-methylpseudourine? 2) Are there any concerns that a DNA vaccine will integrate into host genome in the complete absence of any sort of integrase? 3) Is an immune reaction against ordinary mRNA a problem? Are there any papers documenting instances of this? 4) My biggest question: how is an mRNA with modified nucleosides, a 5' cap, and poly(A) tail manufactured in the quantities necessary to inoculate an entire nation?
Hi Laura,
Original human vaccine for rabies was unmodified and caused rather severe inflammation due to overstimulation of pattern reognition receptors like tlr3, 7, rigi mda5 etc. So the approach taken to make the vaccine acceptable was to incorporate pseudoriridine to dampen the response.
Rna requires a reverse transcriptase to convert it to dna first. Unless the cell is infected with a retrovirus this is highly unlikely as it violates central dogma.
You use a plasmid and transfect a cell line and harvest mrna. Perhaps they ate using oligo synthesizers to get the correct ratio of pseuduuridine incorporation.
- Badges
- Science topic
- Similar topics
- Filing