Kok Wei Xing

4 Questions 3 Answers 0 Followers

Questions related from Kok Wei Xing

Is there a way to isolate CD34 cells from cryopreserved cord blood?

I'm looking for methods to separate CD34 cells from frozen cord blood samples. I'm aware of positive immunomagnetic separation methods, but can anyone recommend alternative such as negative...

05 May 2017 1,617 5 View

How to prepare 5ng/ml PMA from Sigma P8139?

Hi,I'm looking to prepare a working concentration of 5ng/ml of PMA from Sigma's PMA (P8139).How do I go about this? Please advise. I have also provided a link to the relevant data sheet...

22 February 2017 672 2 View

What is the minimum amount of starting material to use in flow-cytometry?

I intend to do CD34 cell sorting so how much cells do I require? Also what is the required amount of proteins fiom these same cells to do disease protein profiling using protein array? Thanks in...

17 October 2016 2,490 3 View

What expression proteomics techniques to use in characterizing the protein profile of iPSC-derived leukemic cells?

I'll be using 2D gel to isolate proteins, but after that I'm not too sure how to proceed (aside from mass-spec). A comparison with actual patient leukemic cells will also be performed. I'd...

07 October 2016 212 1 View