Kok Wei Xing

11 Questions 5 Answers 0 Followers

Questions related from Kok Wei Xing

Is there a way to isolate CD34 cells from cryopreserved cord blood?

I'm looking for methods to separate CD34 cells from frozen cord blood samples. I'm aware of positive immunomagnetic separation methods, but can anyone recommend alternative such as negative...

05 May 2017 1,668 5 View

How to describe HSC and CMP morphology?

Hi,So I will be characterizing hematopoietic stem cells and myeloid progenitor cells using morphological analysis. Has anyone come across such characterization using Wright-Giemsa stain?How do you...

22 March 2017 4,665 1 View

How to prepare 5ng/ml PMA from Sigma P8139?

Hi,I'm looking to prepare a working concentration of 5ng/ml of PMA from Sigma's PMA (P8139).How do I go about this? Please advise. I have also provided a link to the relevant data sheet...

22 February 2017 825 2 View

Which CD markers are best in distinguishing between monoblast from promonocytes in AML-M5 using flow-cytometry analysis?

Hi, So the marker panels I came up with are:monoblast: CD34+ CD64- CD14- CD4- CD38 promonocyte: CD34- CD64++ CD14+ CD117Can anyone confirm or provide better alternatives? Thanks in advance.

21 December 2016 5,501 3 View

Hi, can anyone advise on the differentiation protocols of THP-1 derived iPSC macrophage differentiation?

I will be inducing differentiation of GM colony forming units (from CFC assay) using M-CSF and IL-1beta in medium to yield promonocytes (harvest cells early before complete maturation, I know not...

26 October 2016 2,481 0 View

Does anyone know how to derive promonocytes from hematopoietic CFC Assay?

Hi, I'm doing a study comparing THP-1 cells to differentiated iPSCs. Recently I read that THP-1 cells are actually promonocytes.I'll be utilizing Colony Forming Cell Assay using methylcellulose to...

23 October 2016 4,947 0 View

Is there any substitute to using pHEMA coating in culture flask for macrophage differentiation?

As I understand it pHEMA forms a hydrogel and prevents cell adhesion. I'm trying to induce macrophage differentiation after obtaining myeloid progenitor colonies from CFC Assay. The protocol I'm...

21 October 2016 8,336 1 View

What protocol is best for performing a iPSC hematopoietic differentiation assay?

The type of cells I intend to differentiate are iPSCs generated from THP-1 cell line. I'll also be carrying out downstream characterization of the differentiated cells.Thanks in advance.Wei Xing

20 October 2016 9,275 2 View

What is the minimum amount of starting material to use in flow-cytometry?

I intend to do CD34 cell sorting so how much cells do I require? Also what is the required amount of proteins fiom these same cells to do disease protein profiling using protein array? Thanks in...

17 October 2016 2,601 3 View

What expression proteomics techniques to use in characterizing the protein profile of iPSC-derived leukemic cells?

I'll be using 2D gel to isolate proteins, but after that I'm not too sure how to proceed (aside from mass-spec). A comparison with actual patient leukemic cells will also be performed. I'd...

07 October 2016 282 1 View

How does acid-impregnation affect the carbonization of biomass?

Using 1:6 biomass to acid ratio resulted in a liquefied slurry that wasn't able to dry, and after carbonization it turned into a sticky chunks of carbon. When compared to samples using 1:2 ratios,...

23 November 2014 3,061 6 View