Kenneth Ng

8 Questions 7 Answers 0 Followers

Questions related from Kenneth Ng

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

09 February 2021 6,490 1 View

Can we use the UV from gel doc to do sterilization?

Is it possible to put something in gel doc for UV sterilisation (e.g. 15 minutes) instead of a biological safety cabinet? I once opened the door of a faulty gel doc and accidentally saw the...

11 June 2020 6,425 0 View

Anyone has suggestions on autoclavable 3D printing material for parts for biological research?

I wonder if any of you guys have any experience in producing biological hardwares using 3D printer? What happens if you need it to be sterile? Is there autoclavable 3D printing material? And...

09 June 2020 1,135 0 View

What causes the copy detection limitation in PCR?

Hi all, I wish to understand why doesn't all polymerase can detect a single copy of region of interest? While some polymerase is more sensitive (like dreamtaq), and some are less sensitive, what...

03 June 2020 4,693 21 View

Does anyone has experience with NEB "time-saver enzyme" overnight digestion?

Hi all, I used the Thermo-ANZA series for overnight digestion of plasmid backbone to ensure clean digestion without any problem. How about NEB? The website said all NEB "time-saver enzyme"...

25 March 2020 7,915 2 View

Difference between cell culture plates and flasks?

In the lab we always use culture plates (33, 60, 100, 150mm) for passing cells and conducting all sorts of experiments. I wonder what is the perks of using cell culture plates v.s. flasks? Should...

13 March 2020 1,635 2 View

Can I transfer Spheroid to normal attachment plates for other assays? How will it affect the morphology and consistency?

Do you guys have any experience in transferring spheroid produced in low attachment plate to a high attachment plate after spheroid production? Would it affect the morphology of the spheroid? I...

12 December 2019 2,135 3 View

Various membrane protein expression and functioning in different strain/ organism.

I want to ask whether the membrane protein from different bacterial/Cyanobacteria strains are compatible with each other? For example, can I express all gram positive bacteria membrane protein...

01 January 1970 4,720 0 View