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Questions related from Kenneth Ng
My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....
09 February 2021 6,296 1 View
Is it possible to put something in gel doc for UV sterilisation (e.g. 15 minutes) instead of a biological safety cabinet? I once opened the door of a faulty gel doc and accidentally saw the...
11 June 2020 6,374 0 View
Hi all, I wish to understand why doesn't all polymerase can detect a single copy of region of interest? While some polymerase is more sensitive (like dreamtaq), and some are less sensitive, what...
03 June 2020 4,584 21 View
Hi all, I used the Thermo-ANZA series for overnight digestion of plasmid backbone to ensure clean digestion without any problem. How about NEB? The website said all NEB "time-saver enzyme"...
25 March 2020 7,784 2 View
In the lab we always use culture plates (33, 60, 100, 150mm) for passing cells and conducting all sorts of experiments. I wonder what is the perks of using cell culture plates v.s. flasks? Should...
13 March 2020 1,530 2 View
Do you guys have any experience in transferring spheroid produced in low attachment plate to a high attachment plate after spheroid production? Would it affect the morphology of the spheroid? I...
12 December 2019 2,060 3 View
I want to ask whether the membrane protein from different bacterial/Cyanobacteria strains are compatible with each other? For example, can I express all gram positive bacteria membrane protein...
01 January 1970 4,672 0 View