Jingying Zhou

6 Questions 7 Answers 0 Followers

Questions related from Jingying Zhou

How to explain the inconsistent data from gene overexpression and knockdown experiments?

I am working on gene modulations in cancer cell lines. I found a more obvious transcriptional changes in the gene overexpressed stable cell lines, but marginal changes in gene knockdown cell...

14 September 2021 10,029 2 View

How can I establish mouse liver cancer model by Hepa1-6?

Hi, I am now working on establishing mouse model by Hepa1-6 cell line. 7 days after subcutaneously injection into male C57BL/6J mouse with 5*106 Hepa1-6 cells, solid tumor was established...

15 August 2015 8,977 4 View

What surface marker can be used to identify immature myeloid cell?

I plan to induce MDSCs from immature myeloid cell but not sure about the surface markers to identify immature myeloid cell. Or it is better to use CD34+ cells? Thanks so much for help!

08 April 2015 4,999 3 View

What type of cells co-express CD68 and CD14?

I co-cultured human PBMCs with tumor cell line for three days. Most PBMCs showed double positive for CD14 and CD68. Does anyone know what cell type it is? How about its function? Thanks so much...

08 April 2015 7,254 1 View

Does anyone know of a good kit for isolating genomic DNA and RNA in the same sample?

We sorted cells from patient samples which usually get around 105 target cells only. We want to isolate both DNA and RNA. The Allpre kit from Qiagen resulted in low quantity, about 10-20ng/ul for...

17 March 2015 5,812 14 View

Does anyone know what types of cells could be used for MDSCs differentiation in vitro?

Little confused about MDSCs differentiation and expansion. Could we use immatured myeloid cells sorting from human PBMCs to induce MDSCs differentiation? or it is necessary to use hematopietic...

17 March 2015 1,786 0 View