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Questions related from Jingying Zhou
I am working on gene modulations in cancer cell lines. I found a more obvious transcriptional changes in the gene overexpressed stable cell lines, but marginal changes in gene knockdown cell...
14 September 2021 9,991 2 View
Hi, I am now working on establishing mouse model by Hepa1-6 cell line. 7 days after subcutaneously injection into male C57BL/6J mouse with 5*106 Hepa1-6 cells, solid tumor was established...
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I plan to induce MDSCs from immature myeloid cell but not sure about the surface markers to identify immature myeloid cell. Or it is better to use CD34+ cells? Thanks so much for help!
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I co-cultured human PBMCs with tumor cell line for three days. Most PBMCs showed double positive for CD14 and CD68. Does anyone know what cell type it is? How about its function? Thanks so much...
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We sorted cells from patient samples which usually get around 105 target cells only. We want to isolate both DNA and RNA. The Allpre kit from Qiagen resulted in low quantity, about 10-20ng/ul for...
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