Jayakrishnan .U

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Questions related from Jayakrishnan .U

How to calculate the percentage COD reduction in a fed-batch?

I need to use a fed-batch reactor with continuous feeding for the treatment of wastewater (say 5g COD/L). A 1L reactor having 500 mL culture is being continuously fed with the carbon source,...

08 March 2021 8,806 2 View

What will be the SRT of a SBR with sludge wastage having no settling phase?

As per literature, SRT is estimated according to the equation SRT = Xr*Vr*tc / (Xw*Vw+ Xe*Ve) Xr=VSS content in the reactor (mg/L), Vr= working volume of the reactor (L), tc = cycle length...

18 November 2020 1,001 3 View

Is there any direct HPLC method for quantification of PHB,PHV using C-18 Reverse Phase column?

* Crotonic acid assay is the most rapid and simple method of quantification of PHB. * Karr et al., 1983 showed extension of crotonic acid assay into HPLC for PHB detection. * While Del Don et...

14 January 2020 8,468 2 View

How to make Standard curve for Crotonic acid assay and use it for estimation of Polyhydroxyalcanote from activated sludge?

Hello all, I have been trying to make standard curve for crotonic acid assay from polyhydroxybutyrate-co-valerate (PHBV) dissolved in chloroform. Am facing several issue regarding...

26 March 2019 1,763 1 View

How does sludge retention time of aerobic sequencing batch reactor works?

According to metcalf and eddy, SRT= working volume * MLSS in reactor/(volumetric flowrate* MLSS of sludge return+ volumetric flow rate *MLSS of clarifier effluent). But for SBR sludge return is...

25 September 2018 1,577 3 View

How is valeric acid produced anaerobically from carbohydrates, protien etc., any metabolic pathway?

Among short chain fatty acids, Acetate, propionate and butyrate synthesis are well established anaerobically. But it is hard to find about valeric acid. Does their exist any metabolic pathway that...

03 February 2017 6,650 2 View

Is it possibile to maintain an anaerobic mix culture using defined media?

An Anaerobic sludge from digester consists of various anaerobic organism. Is it possible to use media like Nutrient broth, LB broth, MSM,any such basal minimum media to maintain uniform mix...

06 June 2016 2,541 5 View

Is their any simple method for preparing fatty acid methyl esters from waste water to be analysed in a non polar GC column.?

Acid catalyzed, base catalyzed... every procedure is having the restriction of presence of water. So how could fatty acids present in waste water can be estimated through esterification and...

26 April 2016 9,648 2 View

Can any one suggest a protocol for VFA estimation by GC using DB5ms ultra inert.

I have a non polar column DB5ms ul GC column. But i am unable to find a protocol for standardization and estimation of VFA using the column.

22 April 2016 844 4 View

Which is the best method for the visualization of PHA accumulation in microbial cell?

Sudan Black B, Nile Red/Blue New staining using LipidGreen1

18 August 2015 7,076 3 View