Jayakrishnan .U

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Questions related from Jayakrishnan .U

How to calculate the percentage COD reduction in a fed-batch?

I need to use a fed-batch reactor with continuous feeding for the treatment of wastewater (say 5g COD/L). A 1L reactor having 500 mL culture is being continuously fed with the carbon source,...

08 March 2021 8,776 2 View

Is there any direct HPLC method for quantification of PHB,PHV using C-18 Reverse Phase column?

* Crotonic acid assay is the most rapid and simple method of quantification of PHB. * Karr et al., 1983 showed extension of crotonic acid assay into HPLC for PHB detection. * While Del Don et...

14 January 2020 8,453 2 View

How to make Standard curve for Crotonic acid assay and use it for estimation of Polyhydroxyalcanote from activated sludge?

Hello all, I have been trying to make standard curve for crotonic acid assay from polyhydroxybutyrate-co-valerate (PHBV) dissolved in chloroform. Am facing several issue regarding...

26 March 2019 1,746 1 View

How does sludge retention time of aerobic sequencing batch reactor works?

According to metcalf and eddy, SRT= working volume * MLSS in reactor/(volumetric flowrate* MLSS of sludge return+ volumetric flow rate *MLSS of clarifier effluent). But for SBR sludge return is...

25 September 2018 1,547 3 View

Is it possibile to maintain an anaerobic mix culture using defined media?

An Anaerobic sludge from digester consists of various anaerobic organism. Is it possible to use media like Nutrient broth, LB broth, MSM,any such basal minimum media to maintain uniform mix...

06 June 2016 2,537 5 View