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Questions related from Jayakrishnan .U
I was trying to pretreat rice straw with 5-10 % NaOH at 90 degrees. After that, I used the NREL method (simplified) for lignin estimation as follows: The pH of the pretreated biomass slurry was...
25 November 2024 5,777 0 View
I need to use a fed-batch reactor with continuous feeding for the treatment of wastewater (say 5g COD/L). A 1L reactor having 500 mL culture is being continuously fed with the carbon source,...
08 March 2021 8,869 2 View
As per literature, SRT is estimated according to the equation SRT = Xr*Vr*tc / (Xw*Vw+ Xe*Ve) Xr=VSS content in the reactor (mg/L), Vr= working volume of the reactor (L), tc = cycle length...
18 November 2020 1,056 3 View
* Crotonic acid assay is the most rapid and simple method of quantification of PHB. * Karr et al., 1983 showed extension of crotonic acid assay into HPLC for PHB detection. * While Del Don et...
14 January 2020 8,550 2 View
Hello all, I have been trying to make standard curve for crotonic acid assay from polyhydroxybutyrate-co-valerate (PHBV) dissolved in chloroform. Am facing several issue regarding...
26 March 2019 1,828 1 View
According to metcalf and eddy, SRT= working volume * MLSS in reactor/(volumetric flowrate* MLSS of sludge return+ volumetric flow rate *MLSS of clarifier effluent). But for SBR sludge return is...
25 September 2018 1,647 3 View
I am culturing Ralstonia eutropha in nutrient broth till stationary phase, 60h. Cell pellet lyophilised and then the PHA extraction was tried by two method: CHCl3-60oC for 36h and CHCl3/NaOCl...
19 April 2018 789 0 View
Among short chain fatty acids, Acetate, propionate and butyrate synthesis are well established anaerobically. But it is hard to find about valeric acid. Does their exist any metabolic pathway that...
03 February 2017 6,697 2 View
An Anaerobic sludge from digester consists of various anaerobic organism. Is it possible to use media like Nutrient broth, LB broth, MSM,any such basal minimum media to maintain uniform mix...
06 June 2016 2,590 5 View
Acid catalyzed, base catalyzed... every procedure is having the restriction of presence of water. So how could fatty acids present in waste water can be estimated through esterification and...
26 April 2016 9,689 2 View
I have a non polar column DB5ms ul GC column. But i am unable to find a protocol for standardization and estimation of VFA using the column.
22 April 2016 896 4 View
( APHA 1998) Distillation method or Montgomery method, 1962 Among them which is suitable for rapid detection of Total volatile fatty acid produced during acdiogenic fermentation of waste...
02 March 2016 1,438 3 View
Sewage waste water is my innoculum aeration,nitrogen and phosphorus nutrients are added. my first trail failed. Excessive slurry formed whereby aeration not proper. If some body could help with a...
12 January 2016 8,756 3 View
Sudan Black B, Nile Red/Blue New staining using LipidGreen1
18 August 2015 7,122 3 View
