I have a non polar column DB5ms ul GC column. But i am unable to find a protocol for standardization and estimation of VFA using the column.
Jaya, probably you wont find a GC method using a non polar column for those analytes since the phase of this column has low polarity and works fine with another kind of semi-volatile samples. I would recommend you use a polar column, the best for VFAs is HP-FFAP, it works pretty good (C2-C6) , great resolution and sensibity, however you can use a regular polyethylene glycol phase column.
Other solution could be the alcoholic derivatization of VFAs, not highly recomnended because the losses in the procces but you can try if you can't chose your column.
Regards.
Alexandra
DB-5 is not a recommended column for VFAs. DB-5 is almost a non-polar column ( very little polarity due to 5% Phenyl modification) whereas VFAs are highly polar compounds. You can use a polar column like DB-FFAP where you can inject your VFAs directly without derivatization. The second option is to prepare methyl esters of the VFAs and then you can use a polar wax column like DB-Wax. One of these two options should work out.
Also other good polar columns: DBWax, ZbWax etc. Really, it is a best choice, but unfortuantelly, the durability and stability is rather poor (especially since a view last years). The standard lenght - 30 m or 25 m, 0,25 or 0,32 diameter, 0,25 um film thickness.
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