I have an lenti-transfer vector encoding puromycin resistant gene driven by PGK promoter. I need to select puromycin resistant clones after LV transduction. What is the best way I can select puromycin resistant clones when I am dealing with such a weak promoter?
I am currently doing LV titration with the same LV packaged vector on HEK cells. I know that the protocol I have followed produces lenti viruses are off high titers. However, majority of HEK cells are dying with 10uL of LV.
How can I overcome this problem?