Ima Ghaeli

12 Questions 20 Answers 0 Followers

Questions related from Ima Ghaeli

What is the recommended approach to clean up samples with capto core resin?

Is there any information available regarding the sensitivity of capto core resin to pipetting? Is there a risk of the resin or beads breaking when inserting the pipet tip for sample mixing?...

26 May 2023 8,602 0 View

Is it possible for extracellular vesicles (EVs) containing cancer biomarkers to lack expression of tetraspanin?

Is it possible for extracellular vesicles (EVs) released from cancer cells to contain cancer biomarkers while not expressing tetraspanin (mainly combination of CD9, CD81 and CD63)?

21 May 2023 1,101 8 View

What is the relationship between PEG/Dextran concentration and mixing ratio and the size and concentration of proteins in the ATPS system?

I want to apply PEG/Dextran system to separate specific-sized molecules from a bio-complex system containing various biomolecular components. I would like to know how Dextran concentration and...

09 December 2022 5,136 0 View

What it the most proper immunostaining duration to prohibit self-quenching of antibody conjugated fluorophores?

Hi, I have a specific antibody conjugated alexa fluor 488 to stain tight junction proteins. The free space lifetime is around 4. Based on protocol, I kept cells with stain for an overnight at 4...

01 June 2021 7,858 0 View

Is there any relation between thin film permittivity and nearfield dipole coupling and emission?

Hi, Can anybody help me if various interfaces, metallic and non-metallic thin films, influences the nearfield dipole radiation? I would like to specifically know about the influence of...

28 April 2021 7,354 0 View

What are the main and dominant tight junction proteins existed in the cell membrane of MDCK II, before cell attachment and cell-cell adhesion?

Hi, I would like to know which Tight Junction proteins are more dominant in MDCK cell membranes before starting to attach to surface and growth. Is there any sequential protein recruitment (I...

02 April 2019 1,035 0 View

Is it possible that Crystal Violet passes through the biofilm structure and stains the protein-based substrate beneath the biofilm?

Hi, I used a protein-based substrate to analyze the biofilm growth. The OD results after Crystal Violet staining of substrate before inoculation showed that crystal violet (CV) attached to the...

22 November 2016 8,424 6 View

Is there any relation between biofilm growth and planktonic growth?

Hi, I would like to know that in general, is there any relation between biofilm growth and planktonic growth. Is it possible that biofilm formation and growth reduces the planktonic growth in the...

11 November 2016 8,121 5 View

What is the main reason of polysaccharides binding to T4 TSP?

Hi, I would like to know what is the main reason of phage tail proteins binding to the polysaccharides on the bacterial membrane? Also, is such binding influenced by hydrogen interactions? I...

13 October 2016 6,329 2 View

Can anybody help me with the T4 phage electrostatic charge with and without salts (specially Mg ions)?

I need to know if phage head without salt (specially Mg) may have more negative charge than phage with salt ions. Since Mg ions neutralize the DNA inside capsid, it seems that the capsid should...

16 November 2015 3,794 8 View

How to detect the miscibility parameter in a polyelectrolyte system?

Can anyone help me with the interaction parameter between two oppositely charged polyelectrolytes in a mixture of polyelectrolyte with viscometry technique? I have used Huggins equation and...

23 October 2014 5,033 3 View

How do total and free oxylipin profiles contribute to understanding mechanisms underlying diseases like cancer?

I am seeking information and relevant literature on the comparison between free and total oxylipins across different biological matrices, such as plasma and tumor tissues, particularly in relation...

01 January 1970 5,879 1 View