Difference of species-spesific primers for molecular typing Lactic Acid Bacteria?

05 September 2015 1 6K Report

Hi all

We identified some lactic acid bacteria as lactococcus lactis ssp lactis, lactococcus lactis spp. cremoris and enterococcus faecium with API strips. But we have some problem about molecular identification by standart PCR. Primers targeting 16S for lactococcus lactis spp lactis, also works at cremoris and enterococcus. Also primers targeting cremoris also works at lactis and enterococcus. Is this primer not spesific for this strains or something different that we cant understand clearly.

Best regards

Azad Ismail Saheb

Agree with Michael. We had sequenced B.subtilis 16s and our primers based on the sequence with other strains of B.subtilis. So we have to target areas in the sequence that differ from other strains to have highly specific primers. You need to go for sequencing and then identify areas that differ for designing primers.

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