14 Questions 21 Answers 0 Followers
Questions related from Heba Kamal Issa
i have precipitated enzyme by acetone.. when i use coomassie staining in native gel electrophoresis, it couldnot migrate to stacking and then resolving gel. is there is a problem in acetone...
11 January 2023 2,402 5 View
I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...
05 February 2021 9,400 5 View
I would like to ask about indole production medium composition... I add casein enzymatic hydrolysate or tryptone as tryptophan source... I found both sources were added, for sure one of them...
16 August 2020 7,541 4 View
i have isolates that are gram+ , spore former, catalase and oxidase negative , motile, hydrolyze starch , glucose fermenter, grow at medium containing 10% NaCl and other isolates are non...
01 March 2016 6,794 1 View
i want simple key of physiological tests for identification of halophilic bacteria
01 December 2015 5,589 5 View
i read that 500micro litre of bacterial culture with glycerol 500 micro litre (30%)then in freezer this method is true?? i attach image that show the method >>> if this method is true...
12 August 2015 9,175 12 View
i want procedure for sampling and determination of root exudates especially sugars without HPLC method
27 March 2015 3,714 3 View
i use crystal violet to stain bacterial cells after making film on ordinary slide then count the most obvious bacterial cells .... is this method is true as i donot have epifluorescent microscope
18 March 2015 9,580 6 View
Some times I read the higher is in rhizosphere and some times I read the higher is in rhizoplane... so I want your opinions and causes of your opinion.
20 February 2015 2,429 1 View
From this picture I cannot know if it refer to bacteria death or sporulation (central spore) note this bacteria observed after 24h from incubation. This bacteria isolated from salt marsh. Stained...
13 February 2015 4,352 8 View
On examination of bacteria growing after less than 24h , I found endospore! Is this medium not proper to this bacterium ?
03 February 2015 1,100 8 View
When examined under microscope, bacteria looks like a spiral but there is endospore, so I doubt it is curved bacilli ... I attach an image of the bacteria under microscope
02 February 2015 657 18 View
I would like to determine soil amylase activity at different intervals (5,10,20,30,45) from absorbance (of standard curve).
27 January 2015 8,800 7 View
I want to isolate it from salt marsh which is vegetated with Halophytes
31 January 2014 4,979 3 View
