Heba Kamal Issa

14 Questions 21 Answers 0 Followers

Questions related from Heba Kamal Issa

Why enzymes couldnot migrate from wells to stacking gel in electrophoresis? Is there is problem in its precipitation?

i have precipitated enzyme by acetone.. when i use coomassie staining in native gel electrophoresis, it couldnot migrate to stacking and then resolving gel. is there is a problem in acetone...

11 January 2023 2,386 5 View

What is suitable temperature at reaction mixture of lipase activity assay?

I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...

05 February 2021 9,385 5 View

What is the composition of Indole production medium test for bacterial identification?

I would like to ask about indole production medium composition... I add casein enzymatic hydrolysate or tryptone as tryptophan source... I found both sources were added, for sure one of them...

16 August 2020 7,534 4 View

Is there any Bacillus sp with catalase and oxidase negative in general?

i have isolates that are gram+ , spore former, catalase and oxidase negative , motile, hydrolyze starch , glucose fermenter, grow at medium containing 10% NaCl and other isolates are non...

01 March 2016 6,788 1 View

Does anyone work on identification of halophilic bacteria?

i want simple key of physiological tests for identification of halophilic bacteria

01 December 2015 5,582 5 View

How can I preserve bacterial culture with glycerol but without in liquid nitrogen?

i read that 500micro litre of bacterial culture with glycerol 500 micro litre (30%)then in freezer this method is true?? i attach image that show the method >>> if this method is true...

12 August 2015 9,167 12 View

Any one work on root exudates ?

i want procedure for sampling and determination of root exudates especially sugars without HPLC method

27 March 2015 3,706 3 View

Can I use light microscope with simple stain for bacterial total count?

i use crystal violet to stain bacterial cells after making film on ordinary slide then count the most obvious bacterial cells .... is this method is true as i donot have epifluorescent microscope

18 March 2015 9,572 6 View

In which region microbial count ,diversity and activity are higher: the rhizosphere or the rhizoplane?

Some times I read the higher is in rhizosphere and some times I read the higher is in rhizoplane... so I want your opinions and causes of your opinion.

20 February 2015 2,423 1 View

Does this bacteria produce endospore?

From this picture I cannot know if it refer to bacteria death or sporulation (central spore) note this bacteria observed after 24h from incubation. This bacteria isolated from salt marsh. Stained...

13 February 2015 4,347 8 View

Why do bacteria produce spore after less than 24h of growth?

On examination of bacteria growing after less than 24h , I found endospore! Is this medium not proper to this bacterium ?

03 February 2015 1,095 8 View

Does spiral bacteria produce endoospore ?

When examined under microscope, bacteria looks like a spiral but there is endospore, so I doubt it is curved bacilli ... I attach an image of the bacteria under microscope

02 February 2015 650 18 View

How can I calculate soil enzyme amylase activity?

I would like to determine soil amylase activity at different intervals (5,10,20,30,45) from absorbance (of standard curve).

27 January 2015 8,792 7 View

Anyone working on Halophilic Bacteria? Which medium is used?

I want to isolate it from salt marsh which is vegetated with Halophytes

31 January 2014 4,971 3 View