8 Questions 14 Answers 0 Followers
Questions related from Gustavo Avelar Molina
I am cloning five genes of approx. 1.2 kb each into pET-28a(+) vector (separately, i.e., not all in the same vector) by the USER method. The protocol is essentially the same for all constructs,...
18 February 2023 5,058 8 View
Hi all, I am thinking about other approaches than SEC to separate protein-dye conjugates from free fluorescent dyes, like Alexa, Cy and Atto dyes. Does any of you have experience with that? Will...
18 June 2021 9,225 7 View
I am looking for a reliable and fast method for separation of protein-fluorescent probe conjugates from the respective free fluorophores present in labeling reactions. HiTrap desalting columns...
07 September 2020 8,181 8 View
Dear all, I am preparing a protocol for membrane protein reconstitution in liposomes. In this protocol, the proteins are mixed with the lipids (DLPC), and the liposomes with the proteins are...
01 November 2017 1,620 4 View
I've performed a MD simulation for a protein using GROMACS, and I want to obtain a map for the generalized correlation/dynamic cross correlation in relation to this protein. Note that I'm not sure...
10 January 2017 3,403 6 View
I have constructed a protein-carbohydrate complex based on available 3D structures. Since I did some manual adjustments in the carbohydrate structure, it is needed some kind of energy minimization...
29 October 2016 2,056 3 View
I'm studying the binding dynamics of an enzyme to its substrate by measuring the fluorescence anisotropy of a fluorophore attached to the protein. Since this substrate is a polymer (and/or maybe...
25 February 2015 8,106 4 View
I have purified a protein by affinity chromatography (Ni Sepharose High Performance - GE Healthcare), using a 50 mM phosphate buffer pH 8.0 with 300 mM imidazole and 50 mM NaCl as an elution...
16 February 2015 2,915 14 View
