I want to study the interaction of this polymer with an enzyme that catalyzes its hydrolysis. To know the catalysis kinetics and the hydrolysis degree of a xylan chain in each enzyme-substrate interaction in one molecule level, maybe I will need the presence of a fluorophore in a certain frequency in the polysaccharide structure. There is the EnzChek® Ultra Xylanase Assay Kit, in which the substrate can be excited, but I don't know if the fluorophore is photostable enough to FCS measurements.

To simply measure the enzyme-substrate interaction, maybe probing in one of the chain extremities is enough.