Hi everyone,
Suppose one wants to elucidate the pathway of a plant secondary metabolite, or of a class of plant secondary metabolites (e.g. alkaloids) in a certain organ (e.g. fruit). How should this question be addressed? Where should one start and proceed? Which techniques can be used in each step, and for what reason? There is no need for details on procedures, just the general idea.
It may be a too general question, with perhaps some obvious answers, but it would be really useful for me, who have never worked in this field.
Thank you in advance.
Best Regards,
Gustavo
http://www.pomics.com/li_7_3_2014_158_165.pdf
You can follow this link.. It's a review article.. but probably it can help you...
Hello Gustavo,
Please take a look at the next links:
Plant secondary metabolism databases
http://www.sciencedirect.com/science/article/pii/S2405805X15300156
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4617106/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1104157/
http://nar.oxfordjournals.org/content/34/suppl_1/D511.full.pdf
http://link.springer.com/article/10.1007/s00044-010-9506-z
http://www.ncbi.nlm.nih.gov/pubmed/23185043
http://jgi.doe.gov/first-public-resource-for-secondary-metabolites-searches/
https://omictools.com/secondary-metabolite-biosynthetic-pathways-category
http://www.plantcyc.org/
http://www.plantcyc.org/
http://metacyc.org/
http://www.secondarymetabolites.org/databases/
https://antismash.secondarymetabolites.org/help.html
http://www.genome.jp/kegg/pathway.html
http://metabolomicssociety.org/resources/metabolomics-databases
Through genetics you can elucidate pathways. One way is to screen a mutant population for different contents of the specific metabolite and characterise the mutations in lines with altered levels. Another approach is to screen cultivars for altered levels of this metabolite and use sequencing to link levels to mutations (also works for mutants). Both methods should give you the enzymes and regulators (transcription factors) involved.
