Gurpreet Singh

8 Questions 8 Answers 0 Followers

Questions related from Gurpreet Singh

Can anyone recommend an RNA extraction protocol from Cacao tissue (leaves, meristem, branch)?

Due Covid-19 I personally cannot visit a rural site for the collection of samples. But have found some great people who are happy to do the extractions on my behalf. I need to provide them with an...

20 November 2020 4,132 3 View

Can I do Association analysis (GWAS) on a biparental population that is showing a lot of variation among the genetic distances of the individuals?

I have a biparental population of Cacao for about 188 lines. I was initially thinking of doing QTL mapping, however, when I got the GBS data for my samples, I was informed by the sequencing...

19 February 2020 1,764 3 View

How can I design allele specific primers when I have twp SNPs only few base pairs apart?

Hi there, I am trying to design allele specific primers for KASP assays to validate the SNPs from GWAS results. However, few of my SNP sequences have two or more SNPs only few base pairs apart and...

14 August 2019 9,817 4 View

How do I calculate mean for multi year disease score data for GWAS analysis?

Hi, I have phenotypic data for disease scoring (1-5) over three years for 184 cacao lines. The scoring was done in single environment over a period of three years. To calculate the mean, do I just...

03 August 2019 432 6 View

How can I convert my genotype data file into STRUCTURE input data file for Population structure analysis?

I have genotype data scored as 0 and 1 for presence/absence of marker in the hapmap format. How can I convert it into input format for STRUCTURE software for Population structure analysis? Please...

04 June 2019 3,364 1 View

How can I prevent the degradation of the DNA?

I have extracted DNA from cacao leaf tissue (freeze dried). I used CTAB buffer (5%) along with 1% B-mercaptoethanol and 3% PVP. I did the DNA extraction overseas and transported it as a pellet to...

17 January 2019 2,154 12 View

Why am not seeing any DNA bands from my samples?

I have extracted DNA from Cacao leaf tissue using CTAB, chloroform isoamyl alcohol method. When I quantified my samples on Nandodrop they show a conc. of 300-800 (ng/ul) in 50 ul of TE buffer. The...

16 January 2019 2,232 14 View

Is it okay If I donot remove RNAse from my DNA samples after treating it for an hour at 37 C?

I am trying to remove RNA contamination from my DNA samples. I gave RNAse treatment for DNA resuspended in 50 ul of TE buffer. After 1 hour of treatment I reprecipitated the DNA using 1/10 V of...

15 January 2019 5,876 2 View