Cell lysis protocol for Picogreen DNA assay?

I'm trying to quantify the amount of DNA in adherent cells, does anyone have a good protocol for cell lysis to prepare samples for Picogreen DNA assay? Any help will be greatly appreciated! Gloria

04 May 2016 1,438 2 View

Can you measure amount of beta-tubulin protein with respect to beta-actin?

Both beta-actin and beta-tubulin are frequently used as load controls in WB, but can I measure the amount of beta tubulin in my sample and normalized with respect to beta-actin? In other words,...

04 May 2016 2,367 2 View

What type of brain cancer cell line is most vulnerable to T-cells?

I heard most brain cancer cells avoid t-cells very well, but is there a cell line (human or animal) that is a little more vulnerable to T-cells than others? thanks! Gloria

07 August 2015 446 3 View

How do you keep PC12 cells separate from each other?

PC12 cells easily form clumps, is there a way to prevent this clumping ? Thanks, Gloria

06 July 2015 6,563 2 View

How do you get subclonal of differentiated PC12 cells?

how do you get subclonal cell line of well differentiated PC12 cells? Thanks!

06 July 2015 1,542 2 View

Is there a way to transfer live cells, without freezing them for several hours?

Other than using a portable incubator, is there a way to transfer cells without freezing for many hours?

04 May 2014 3,444 14 View

What is a solvent for folic acid?

What is a solvent for folic acid?

04 May 2014 1,893 16 View

Can you get >100% cell viability in OD readings using MTT assays and other such assays?

I would like to know if you can get more than 100% cell viability using MTT assays. Thanks!

02 March 2014 308 6 View

Can anyone help with non-surface markers for THP1 cells?

What are good non-surface markers that I can use to characterize differentiated THP1 cells?

02 March 2014 6,977 3 View

What's good as a pre-coater for better platinum adhesion on SiO2

Any material good for enhancing platinum adhering on hydrophobic surfaces?

05 June 2013 7,999 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View