Color at the test line of a lateral flow imuunoassay strip ?

Hi, I am trying to develop a competitive lateral flow assay to detect a protein. I coat the protein on the test line and use gold nanoparticle-antibody as the conjugate that binds to the test...

14 February 2019 2,153 4 View

What is the characteristic UV-Vis Spectrum of Ultra small Gold Nanoparticles ?

Hi, I am trying to synthesize ultra small 2nm gold nanoparticles by NaBH4 reduction (Brust Method). What should be the characteristic UV-Vis spectrum of 2nm size gold nanoparticles. Should we or...

18 September 2018 4,460 9 View

What are the ways to inactivate a Virus without affecting its ability to bind to its antibody ?

We are trying to detect a Virus using a sandwich lateral flow assay. We are targeting the epitopes on the envelop protein of the virus. Is there anyway I can inactivate the disinfect the virus,...

15 March 2018 2,631 3 View

How much more difficult it is to detect a whole virus when compared to its Non Structural (NS) Protein ?

I am trying to develop a gold nanoparticle based lateral flow sensor to detect a whole intact Virus. I have tried with 3 different antibodies of the virus. But, I was not successful. Reading the...

08 February 2018 3,611 8 View

Is there any ideal initiator for synthesis of PMMA Nanoparticles through emulsion radical polymerisation ?

I would like to know whether ammonium per sulfate (APS) is a good radical initiator for synthesizing PMMA nanoparticles by emulsion radical polymerisation. Or, does it have any drawbacks...

04 October 2016 7,812 8 View

What Quantum dots would be ideal for highly sensitive paper based lateral flow assay?

Can anyone who worked with quantum dots suggest what be the ideal quantum dot to use for paper based lateral flow assay. I should be able to conjugate antibody to it and we are looking for a...

17 August 2016 7,636 3 View

Can anyone help me with designing a portable sensor using a nitrocellulose membrane ?

I need to develop a portable low cost sensor using a nitrocellulose membrane (NM). I have done an assay for my analyte on a 96 well plate using monoclonal and polyclonol antibodies and a labeling...

31 May 2016 9,650 1 View

Any advice for absorption spectrum coming in the negative region of the y-axis in a UV-vis spectrophotometer?

In a UV-vis apectrophotometer, what does it mean if the absorption spectrum is coming in the negative region of the y-axis (absorbance) even after taking a baseline with reference?

25 December 2013 3,859 21 View

Can anyone help me with design of paper strip for lateral flow immunoassay ?

I have to develop a paper strip for detection of a small molecule, a nucleoside. I need some suggestions on the arrangement of different pads as I am doing the paper based sensor for the first...

01 January 1970 4,405 7 View

Is Lateral Flow Assay specific to clonality of the anitbody ?

01 January 1970 7,976 12 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View