Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

When i accept card in "HighScore plus app" should i attention to cards quality (in Reitveld analysis)?

Hi I wanna use Reitveld analysis in my project but i have big question that is: Should i see quality of each card (or Not important !!?), or it is enough to check the score and scale factor of...

04 January 2021 7,750 1 View

How much the microbial exploration in oil and gas industry is applicable?

Microbial oil and gas prospecting (MPOG) I would grateful it if you could provide me with your invaluable comments and ideas on the following questions: 1- Do you consider MPOG as a promising...

15 December 2020 9,895 2 View

Does any one can help me about my irc error messages in gaussian?

Hello every body I am using IRC command line to confirm my ts molecule which is obtained from Scan. my problem is that some of my irc files ran abnormally I mean when I open it with gauss view the...

13 December 2020 7,955 5 View

Monte Carlo Simulation, JMP, Minimum Sample Size of a SEM?

22 November 2020 7,626 2 View

Did anybody know about effect of aldehyde on spidermite?

What organic acids influence on spider mite?

11 October 2020 9,944 3 View

How can technology impact on agriculture section of India? How can startups help to apply technology in agriculture of India?

Dear Researchers, I am working on a project about Agri-tech startups in India. Any information or work experience related to Agricultural-technology startups would help me in my project. I also...

17 September 2020 6,059 4 View

HiPSC positive for GLAST in FACS - why?

Hey, I'm working on hiPSC differentiation into astrocytes. I stained my cells (hiPSC stage) with a GLAST (ASCA1) antibody for FACS and the cells showed a strong positive signal. As far as I know,...

09 February 2021 3,383 4 View

What is a suitable method to freeze feeders-free, serum free episomal hiPSC?

Hi everyone, I need your professional advice: I started growing episomal iPSC (commercial cell line). I grow them in StemFlex medium. What would you recommend as the most effective freezing...

07 February 2021 8,591 4 View

Ac16 cardiomyocytes cell line culturing ?

I have faced a problem with culturing Ac16 cardiomyocytes cell line. Day 0 I plated 200,000 cells/2ml (well) in 6 wells plate in FGM. Day 3 I put my cells on Low serum medium. When I changed...

24 January 2021 1,456 2 View

Is there any method I can use to isolate single cells (i.e cardiomyocytes and non-cardiomyocytes) from snap from rat heart tissue?

I have GD18.5 heart tissue currently snap frozen in -80, apart from mRNA and protein analysis on whole tissue, is there a way of isolating single cell populations to study localization of a...

19 January 2021 4,044 3 View

Are these IPScs Differentiated ?

Hi everyone, So these are images of IPSCs 2 days post passaging/splitting. I noticed that the overall morphology of colonies are not compact or round and without distinct border. However, at...

28 December 2020 3,503 3 View

How to isolate human cardiac stem cells? Which other cell types could be in this cell culture?

Hello, I followed a protocol for isolating human cardiac stem cells (Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human...

18 November 2020 2,928 3 View

How to stain iPSC derive motor neuron in 96-well plate without detachment?

Hi all, I am using 96-well plates to culture iPSC derived neurons, the problem is, when doing ICC, my neurons tend to detach during the washing procedure, till the final step nearly all neurons...

14 July 2020 3,828 7 View

Why does my H9 IPSC die reasonless after 80% confulency?

Dear All, I am working with thermo fisher H9 cell line, human induced pluripotent stem cell. I made a mutation on them with crispr cas-9 I obtain single colonies and I propagate my clones. I...

13 June 2020 9,046 3 View

Are there reliable antibodies against TRP channels in cardiac cells ?

From the literature I understand that TRP channels regulate Ca2+ signalling in cardiomyocytes and fibroblasts. I want to perform immunocytochemical analysis of TRP channels on cultured cells or...

07 May 2020 6,865 1 View

How to resuspend cultured adult rat myocytes without damaging the cells?

Hello everyone, Which is the best way to resuspend a culture of adult rat ventricular cardiomyocytes without damaging the cells integrity? This is what I am trying to do. I isolate ventricular...

31 December 2019 1,080 2 View