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Questions related from Fadaka Adewale, Oluwaseun
the Patch Dock is giving me trouble regarding the code for my ligand the error message is " Error in receptor molecule input: please specify PDB code or upload file " My ligand is microRNA. and it...
06 June 2019 5,166 3 View
Attached below is the second message i received from Lambert Academic publishing. although the offer is kind of enticing but i do not want to jump into less reliable publishers. Please assist...
09 September 2018 7,814 0 View
Any best way to troubleshoot qPCR, working currently on qPCR but when the gel is run, all i see is shadows and no distinct band, What variable should i change and also is two parameters...
05 May 2018 8,700 0 View
I am new to Biotechnology and my area of PhD research now is Bioinformatics. I find it very hard to run smoothly most of the software given to me to carry out biology result. Can anyone suggest...
04 April 2018 5,200 4 View
Dear all, I want to extract the genes related to colorectum but the database TIGER only showed the tissue view of colon. please suggest any database that can give the ESTs or expression profile of...
04 April 2018 1,127 8 View
I need to identify some targets of a number of miRNAs but i need suggestions on the best target prediction tool or a combination majorly for humans. Thanks
04 April 2018 7,091 3 View
Hello all, i am trying to compare the results of blast to cd hit est 2d. the initial run gave 15 and 10 respectively but the common miRNAs between them is 6. I want to increase the miRNAs...
04 April 2018 1,915 2 View
Can BLAST and CD-HIT be regarded as text mining process in bioinformatics or are there special or specific tools used to text mine data ?
04 April 2018 4,001 0 View
In invitro studies, is it feasible using neuronal stem cell model to investigate the mechanism of how anti-psychotic drugs affect neuronal development, can this group suggest any previous work...
10 October 2017 10,078 3 View
Does gluconic acid react with ferricyanide or Ferricyanide is used as an electron mediator between the enzyme and the electrode. ? or can someone clearly explain the principle or refer an article
01 January 2017 2,858 0 View
although it is not well known to the world about Necrotizing fasciitis but the modified form of this bacteria can be a good anti cancer agent. are there present work or reviews on this ?
08 August 2016 8,836 2 View
can anyone really point out the relationship between fold change, efficiency and threshold cycle number as a method in gene expression determination. thanks
06 June 2016 4,126 4 View
One miRNA could have several targets, but these targets may not all be regulated by the miRNA in every circumstance. For standard utilization of miRNAs as biological function and to prevent the...
04 April 2016 7,684 2 View
Dear researchers, i need some cost effective protocols concerning the evaluation of internal controls for qualification of Mi-RNAs, validation of Mi-RNA markers and quantifying Mi-RNAs using...
03 March 2016 325 3 View
What are the required reagent and perfect procedure to ISOLATE MICRO-RNA with self made cost effective reagent. previous work don on the isolation and characterization can be suggested or attached...
03 March 2016 622 4 View
In PCR optimization, What is the best annealing temperature to prevent primer-dimer
03 March 2016 5,560 3 View
is gantt chat an application or its from microsoft excell, how do i best use it to plan for my work ?
02 February 2016 1,836 0 View
I'm presently working on microRNA against colorectal cancer in UNILAG for my PhD and i really need supporting related review and research articles for this work. suggesting articles are allowed....
01 January 2016 5,567 0 View
Can anyone suggest the easiest way to blast a given sequence aside the idtdna.com. I really need to learn more about blasting. Thanks
01 January 2016 2,743 3 View
Can anyone please suggest the standard procedures for the analysis of fat and water soluble vitamins in plant extracts? thanks in anticipation .
10 October 2015 6,807 0 View
Why do air bubbles inhibit polymerization
09 September 2015 5,794 0 View
I just wonder even after following al the protocols and preparing the stacking gel, it will only form a semi-solid gel. And refuse to solidify like the loading gel why?
09 September 2015 9,977 4 View
Can someone assist with the following topics. 1..ENZYMATIC BROWNING, 2..Genetically Modified Organiganism (GMO) in food; biochemistry and application. 3...food enzymes and their applications in...
09 September 2015 9,622 4 View
How do i sequence DNA manually using Sanger's method, a protocol will help. thanks. A full procedure is highly needed
08 August 2015 9,116 0 View
What determines the size of the DNA ladder ?
08 August 2015 5,162 1 View
I will be carrying out ELISA out in my research work and i dont want to rely on the protocol i have. I want to ask that you pls assist my in any already carried out ELISA protocal without any...
08 August 2015 4,664 1 View
Thanks to this platform, I really need a standard sample of PhD proposal writing guideline to follow, attached sample is welcome. thanks as you assist
08 August 2015 2,002 2 View
Regarding the radio and magnetic waves emitted by mobile phones, has there any being any concrete evidence to prove that they cause cancer or enhance tumour formation, any mechanism of action ?...
08 August 2015 4,078 0 View
I was to carry out a research in our lab and the first step is to purify protein extracted from the sample and check bands using SDS-PAGE. The procedure used for the resolving gel are as follows;...
08 August 2015 9,684 4 View
Sciences, Geneticist, Molecular Biologists and Biotechnologists
08 August 2015 231 1 View
it is said to be important to determine sample size in any research study, does sample size determination vary between designs and can anyone suggest the easiest way to calculate/determine the...
01 January 1970 5,297 7 View
I am sure if the binding site of a microRNA can be implicated on a gene or mRNA there one can conclude about the mechanism of action and in a cancer case, its parthenogenesis. Is the binding on...
01 January 1970 8,148 5 View
There are numerous bioinformatics tools nowadays which predict the targets of a given miRNA. Is there any tool/ software to find the microRNAs regulating a mRNAs and is there a rule guiding...
01 January 1970 1,844 3 View