11 Questions 63 Answers 0 Followers
Questions related from Ekaterina Chesnokova
Hello colleagues, I feel like it's a stupid question but I couldn't google the answer quickly. Are there any conventional rules about multiple sequence alignment visual presentation - sequences'...
02 February 2019 4,328 1 View
Hello everybody, I'm preparing stock aliquots of cell culture supplements, and I have a problem dissolving insulin. The old protocol in my lab that I tried to use first says to dissolve insulin in...
01 January 2019 4,506 1 View
Hello, I have a new batch of Hardset Vectashield that I forgot to put in the fridge one day and it spent a night on the table. Now I used it to mount/counterstain some cultures and the result...
11 November 2018 5,270 4 View
Hello colleagues, I'm still new to cell culturing so I'm really paranoid about contamination. I found these worm-like fibers in some of my cell culture flasks. I attach photos here. The cells are...
10 October 2018 4,400 4 View
Hello colleagues, I recently had much unspecific staining in my immunofluorescence assay. The blocking solution we use is not sterile and contains three different sources of protein in high...
09 September 2018 3,448 7 View
Hello colleagues, I have one dubious pair of primers for a snail gene which efficiency is very unstable and can oscillate from 80 to 110% in different reactions (standards are not to blame,...
08 August 2018 716 3 View
Hello everybody, I worked with qPCR using SYBR Green for detection, so primer-dimers were a real bane for me. I tried to design my primers carefully, checked them for homo- and heterodimerization...
08 August 2018 5,362 4 View
Hello colleagues, I am sure that someone had already asked this before more than once, but I couldn't find similar questions quickly. I have a PhD and have been working in science for a few years,...
08 August 2018 6,905 4 View
Hello! I'm trying to perform an optimization of qPCR for four genes of snail's nervous system (two isoforms of the same kinase and two housekeeping genes). I designed primers, checked them in...
12 December 2016 8,613 29 View
Hello everybody, I prepare samples for RNA-Seq using Ion Proton semiconductor sequencer. I can generally assess cDNA libraries construction and chip loading, but I know little about...
09 September 2016 602 14 View
Hello everybody! Not long ago I learned to do SDS-PAGE and Western-blotting, these methods are still quite new for me. I noticed a peculiar detail about my recent blots: the molecular mass marker...
02 February 2016 9,161 8 View
