Edwin Velazquez

9 Questions 11 Answers 0 Followers

Questions related from Edwin Velazquez

Does anyone know how to blast a mouse VH sequence against all human VHs contained in the IMGT database?

I have the sequence of a mouse antibody and want to know which are the closest related human VH sequences by percentage of similarity or alignment to my murine VH sequence. I found all the IGHV...

19 August 2021 6,101 4 View

Anyone experienced in using Dab library from Daniel Christ?

I have been troubleshooting the use of this library for the isolation of antibody fragments for a couple of months with some success.However I went into some troubleshooting. I'm looking for...

17 June 2019 8,529 3 View

Has the sandwich ELISA kit (LSBIO,F11880) for quantification of TK in human serum worked for anyone?

I'm trying to see if someone has quantified human Thyimidine Kinase 1 using this kit or if the kit has been quoted in a paper.

12 March 2019 6,658 1 View

Why my TG1 bacteria is growing in my TYE Kanamycin plates if it is not infected with helper phage?

I've been growing TG1 bacteria in M9 minimum salt plates for 36 hrs. picking a colony and grow it in 2xTY media overnight. Next day I'm starting a 100-fold diluted culture and growing it until it...

06 September 2018 4,874 10 View

Why am not getting plaques in my serial dilutions, during my helper phage amplification protocol?

I've been trying to amplify KM13 helper phage using the protocol in box one from the Christ Dab library. I've been doing serial dilutions from the phage stock, infecting 200 ul TG1 bacteria at...

14 August 2018 4,156 4 View

Are there human anti-rabbit and human anti-mouse secondary antibodies that could be used for in vitro ADCC experiments?

Am trying to run an ADCC experiment using human NK (NK-92 and primary) cells and a phagocytosis assay with human macrophages (THP-1 and primary). However my antibodies against my surface antigens...

09 August 2018 3,279 4 View

My hybridomes have been dying, How to grow hybridomes which have been stored for 10 years?

Hello guys, I'm trying to grow some hybridomes which were frozen 10 years ago, I have been trying to grow them in RPMI + 10 % of FBS, but after one week they start to die, do you have some...

08 February 2016 9,824 4 View

Dos anyone know where I can find a protocol to induce protein expression in yeast using GAL1-GAL10 promoter?

Trying to produce human proteins in yeasts

09 December 2015 960 4 View

I'm trying to make a 2M solution of MgCl2 to make competent cells, but a precipitate is formed. Is that normal, if not what can I do to dissolve it?

Making ultra competent cells with the Inoue method

05 December 2015 3,688 6 View