Edwin Velazquez

5 Questions 8 Answers 0 Followers

Questions related from Edwin Velazquez

Does anyone know how to blast a mouse VH sequence against all human VHs contained in the IMGT database?

I have the sequence of a mouse antibody and want to know which are the closest related human VH sequences by percentage of similarity or alignment to my murine VH sequence. I found all the IGHV...

19 August 2021 6,095 4 View

Anyone experienced in using Dab library from Daniel Christ?

I have been troubleshooting the use of this library for the isolation of antibody fragments for a couple of months with some success.However I went into some troubleshooting. I'm looking for...

17 June 2019 8,510 3 View

Has the sandwich ELISA kit (LSBIO,F11880) for quantification of TK in human serum worked for anyone?

I'm trying to see if someone has quantified human Thyimidine Kinase 1 using this kit or if the kit has been quoted in a paper.

12 March 2019 6,641 1 View

Why my TG1 bacteria is growing in my TYE Kanamycin plates if it is not infected with helper phage?

I've been growing TG1 bacteria in M9 minimum salt plates for 36 hrs. picking a colony and grow it in 2xTY media overnight. Next day I'm starting a 100-fold diluted culture and growing it until it...

06 September 2018 4,868 10 View

I'm trying to make a 2M solution of MgCl2 to make competent cells, but a precipitate is formed. Is that normal, if not what can I do to dissolve it?

Making ultra competent cells with the Inoue method

05 December 2015 3,684 6 View