11 Questions 6 Answers 0 Followers
Questions related from David Philpott
Hi, I have a microfluidic channel with a very low aspect ratio (100 um tall x 2 mm wide). When I flow cells though this channel I notice cells move at two separate speeds. I am trying to figure...
13 October 2020 5,556 2 View
I am trying to dice a 4" wafer made from Gorilla glass ( McMaster Carr 8410T3) using a Disco DAD3220 wafer dicing machine. I am having trouble getting a clean cut. When I set the blade to cut...
01 August 2019 7,398 3 View
I have designed a microfluidic chip made from SU8 3010. Quite often cells will get stuck running along the surface of the SU8. I have also notice that under FITC conditions, chips that fail tend...
30 July 2019 1,442 0 View
Hi, Is there a good database for immortalized human cell lines? In particular cancer cell lines? Many times I need to look up attributes of a cell line; size, protein expression, doubling time,...
05 June 2019 1,197 5 View
I am trying to 3D print a microfluidic device on glass using a mixture of poly(ethylene glycol) diacrylate (MW 250) + 1% w/w Igracure 819 ( Bis(2,4,6-trimethylbenzoyl)-phenylphosphineoxide) and...
25 May 2018 127 2 View
Hello, I would like to bond 3D printed PEG-DA 250 to a glass slide, (similar to creating soft-lithography devices with PDMS). Any suggestions on how to do this? I have tried an O2 plasma but it...
27 April 2018 4,606 6 View
Hello, I am spin coating PDMS over a glass wafer with patterned metal. It is important to me that this PDMS surface is smooth. Is there an easy way to measure the surface roughness? I was...
21 March 2018 8,505 4 View
For a microfluidic device of mine, cells are sticking to a PDMS surface (not oxidized). Are there any surface treatments that can be used to reduce this effect? It may also be that the surface of...
18 March 2018 6,260 5 View
Hello, For a microfluidic device I have built, I have a layer of PDMS acting as a gasket. I would like to be able to sterilized this device such that it can be reused. I am aware of multiple...
18 March 2018 8,083 4 View
Hello, I am looking to order some photosensitive polyimide precursor for the manufacture of microfludic devices. But I ma having trouble finding good suppliers. I have contacted HD Microsystems,...
21 June 2016 1,953 2 View
Hello All, This may be a very straightforward question, but I am new to this field and things might not be obvious to me. But I am looking to use a piranha solution to remove SU-8 after is has...
18 May 2016 3,648 1 View