David Philpott

11 Questions 6 Answers 0 Followers

Questions related from David Philpott

Cells Moving at Two Speeds in PDMS Microfluidic Device - how to resolve?

Hi, I have a microfluidic channel with a very low aspect ratio (100 um tall x 2 mm wide). When I flow cells though this channel I notice cells move at two separate speeds. I am trying to figure...

13 October 2020 5,561 2 View

Have you diced a Gorilla glass wafer?

I am trying to dice a 4" wafer made from Gorilla glass ( McMaster Carr 8410T3) using a Disco DAD3220 wafer dicing machine. I am having trouble getting a clean cut. When I set the blade to cut...

01 August 2019 7,408 3 View

Is autofluorosence of SU8 and indicator of underdevelopment?

I have designed a microfluidic chip made from SU8 3010. Quite often cells will get stuck running along the surface of the SU8. I have also notice that under FITC conditions, chips that fail tend...

30 July 2019 1,447 0 View

Database for immortalized human cell lines?

Hi, Is there a good database for immortalized human cell lines? In particular cancer cell lines? Many times I need to look up attributes of a cell line; size, protein expression, doubling time,...

05 June 2019 1,212 5 View

Curling of PEG-DA when exposed to UV light?

I am trying to 3D print a microfluidic device on glass using a mixture of poly(ethylene glycol) diacrylate (MW 250) + 1% w/w Igracure 819 ( Bis(2,4,6-trimethylbenzoyl)-phenylphosphineoxide) and...

25 May 2018 132 2 View

Bonding PEG-DA 250 to glass?

Hello, I would like to bond 3D printed PEG-DA 250 to a glass slide, (similar to creating soft-lithography devices with PDMS). Any suggestions on how to do this? I have tried an O2 plasma but it...

27 April 2018 4,614 6 View

Simple way to measure PDMS surface roughness?

Hello, I am spin coating PDMS over a glass wafer with patterned metal. It is important to me that this PDMS surface is smooth. Is there an easy way to measure the surface roughness? I was...

21 March 2018 8,514 4 View

What simple surface treatments can be used to prevent cells from sticking to PDMS?

For a microfluidic device of mine, cells are sticking to a PDMS surface (not oxidized). Are there any surface treatments that can be used to reduce this effect? It may also be that the surface of...

18 March 2018 6,269 5 View

What material can act as a microfluidic gasket AND can withstand being autoclaved? Can PDMS be autoclaved?

Hello, For a microfluidic device I have built, I have a layer of PDMS acting as a gasket. I would like to be able to sterilized this device such that it can be reused. I am aware of multiple...

18 March 2018 8,090 4 View

What are the best suppliers for photosensitive polyimide (PSPI)?

Hello, I am looking to order some photosensitive polyimide precursor for the manufacture of microfludic devices. But I ma having trouble finding good suppliers. I have contacted HD Microsystems,...

21 June 2016 1,960 2 View

What are the effects of a piranha solution on deposited copper?

Hello All, This may be a very straightforward question, but I am new to this field and things might not be obvious to me. But I am looking to use a piranha solution to remove SU-8 after is has...

18 May 2016 3,657 1 View