David Bovard

6 Questions 8 Answers 0 Followers

Questions related from David Bovard

How to obtain thick 3D oral tissues (keratinocytes)?

Dear all, I have been trying for a while to generate 3D oral tissues from primary human keratinocytes cultures at the air-liquid interface with various calcium concentration (0.5 - 1.5 mM) in the...

19 August 2024 3,041 0 View

Any goblet and ciliated cell extracellular marker?

Dear All, Would any of you know an extracellular markers to target specifically ciliated and goblet cells from bronchial tissues? Thanks

14 January 2016 2,321 4 View

Can you suggest any publication dealing with changes in channel subunit stoichiometry after knock down of a subunit?

Hello, I'm actually looking for a publication in which they demonstrate that in a heteromeric channel formed of two subunits A & B, if they knock down A for example, then the channel will...

02 December 2014 1,098 0 View

How do you detect Ankyrin repeats in a given amino acid sequence?

Hi everybody, I think everything is almost in the title of topic. I'd like to know how is it possible to know if my amino acid sequence contains ankyrin repeats? I know predicting programs for...

05 August 2014 1,492 2 View

Can anyone help with a problem with Ni-NTA pulldown?

I'm facing a strange problem with my Ni-NTA pull-down. So I transfect my HEK cells with one plasmid having a His tag (Protein tagged) and one other protein without the tag (protein B) and I'd...

07 March 2014 6,632 17 View

Problem with transfer of a 6% PAGE gel?

I'm trying actually to observe a protein of 350 kDa. In order to see this protein I have first separated my protein on a standard 6% PAGE gel. Then I have tried to transfer the protein on a...

26 November 2013 7,763 5 View