Dan Naylor

6 Questions 2 Answers 0 Followers

Questions related from Dan Naylor

How to assay Bacillus thuringiensis microbial isolates for toxin production?

Our group has been having difficulties finding a consistent mechanism by which to screen Bt isolates for Cry or Cyt toxin production in a quantitative, reasonably high-throughput manner. Does...

22 May 2023 276 0 View

How to culture Gram-positive bacteria from soil (and NOT Gram-negative bacteria)?

I'm wondering, if you have a bulk soil or rhizosphere soil source, what kinds of media, substrates, conditions, etc. you might use to specifically target Gram-positive bacteria at the expense of...

18 August 2022 4,001 3 View

What kinds of soils are rich in nitrogen-fixing bacteria?

E.g. is there a particular soil trait (pH, chemical content, concentrations of carbon or nitrogen, loamy vs. sandy, etc.) that is associated with higher populations of diazotrophs? I'm more...

14 January 2022 1,402 4 View

Why is my nitrogen free media coming out way too basic?

Hi, I'm making up a few different types of nitrogen-free media, including nitrogen-free malate with bromothymol blue (NFb) and nitrogen-free combined carbon (NFCC) media. NFb medium composition...

03 December 2021 5,751 3 View

Microbiome analysis - how to determine correlations between individual taxa abundance and levels of categorical variables?

Here's my situation: I'm doing an analysis of soil communities under 5 different irrigation regimes (100%, 75%, 50%, 25%, 0% moisture) and 3 different soil depths (surface, immediate subsurface,...

28 March 2021 5,375 2 View

PEG agar plates

Hi, I'm looking for some clarification on how to make PEG-agar plates. In the protocols I've looked at, they've mentioned using filter paper or nylon mesh in between the solid, PEG-less agar and...

03 July 2017 5,442 2 View