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Questions related from Christopher J Vavricka
We are compressing high dimensional vectors to 2 dimensions using PCA, so that we can make 2D SVM plots. But it is difficult to compress to 3 dimensions with PCA.What is a good way to compress to...
02 February 2021 6,805 6 View
Can araBAD vectors be induced by arabinose in BL21-AI (F-ompT hsdSB (rB- mB-) gal dcm araB::T7RNAP-tetA), or in Lemo21(DE3) (fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS/ pLemo(CamR) λ DE3 = λ...
31 January 2020 595 3 View
I am new to the Shimadzu GCMS-QP2010 Ultra. The machine passed a system check report, and then I tried testing acetone injection with a DB-5 column. After ~2-3 minutes, when the column temperature...
31 July 2019 8,891 3 View
Most proteins should be denatured after autoclaving growth medium containing yeast extract, but I wonder if there are any reports about active yeast enzymes in growth medium?
30 July 2019 9,399 4 View
The following is a list of functional groups in decreasing order of their negative inductive effect (-I): —NH3+ > —NO2 > —SO2R > -CN > -SO3H > -CHO > CO > —COOH > -COCl>...
28 September 2017 582 0 View
I am working on several unrelated enzymes that produce toxic products. All genes have been cloned into T7 promoter containing expression vectors, yet when transforming to non-expression hosts such...
18 September 2017 7,913 7 View