Christopher J Vavricka

3 Questions 8 Answers 0 Followers

Questions related from Christopher J Vavricka

Is there an E. coli host that be used with an araBAD vector together with a T7 vector?

Can araBAD vectors be induced by arabinose in BL21-AI (F-ompT hsdSB (rB- mB-) gal dcm araB::T7RNAP-tetA), or in Lemo21(DE3) (fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS/ pLemo(CamR) λ DE3 = λ...

31 January 2020 557 3 View

How to fix Shimadzu GCMS-QP2010 CAR1 purge leaks?

I am new to the Shimadzu GCMS-QP2010 Ultra. The machine passed a system check report, and then I tried testing acetone injection with a DB-5 column. After ~2-3 minutes, when the column temperature...

31 July 2019 8,618 3 View

How can I optimize growth of E. coli transformed with a toxic gene?

I am working on several unrelated enzymes that produce toxic products. All genes have been cloned into T7 promoter containing expression vectors, yet when transforming to non-expression hosts such...

18 September 2017 7,880 7 View