Christopher J Vavricka

6 Questions 11 Answers 0 Followers

Questions related from Christopher J Vavricka

How to compress high dimensional support vector machine (SVM) to 3 dimensions?

We are compressing high dimensional vectors to 2 dimensions using PCA, so that we can make 2D SVM plots. But it is difficult to compress to 3 dimensions with PCA.What is a good way to compress to...

02 February 2021 6,814 6 View

Is there an E. coli host that be used with an araBAD vector together with a T7 vector?

Can araBAD vectors be induced by arabinose in BL21-AI (F-ompT hsdSB (rB- mB-) gal dcm araB::T7RNAP-tetA), or in Lemo21(DE3) (fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS/ pLemo(CamR) λ DE3 = λ...

31 January 2020 605 3 View

How to fix Shimadzu GCMS-QP2010 CAR1 purge leaks?

I am new to the Shimadzu GCMS-QP2010 Ultra. The machine passed a system check report, and then I tried testing acetone injection with a DB-5 column. After ~2-3 minutes, when the column temperature...

31 July 2019 8,961 3 View

Can autoclaved yeast extract retain any enzymatic activity?

Most proteins should be denatured after autoclaving growth medium containing yeast extract, but I wonder if there are any reports about active yeast enzymes in growth medium?

30 July 2019 9,407 4 View

Does a salt bridge interaction with an electronegative group increase or lower its electronegativity?

The following is a list of functional groups in decreasing order of their negative inductive effect (-I): —NH3+ > —NO2 > —SO2R > -CN > -SO3H > -CHO > CO > —COOH > -COCl>...

28 September 2017 589 0 View

How can I optimize growth of E. coli transformed with a toxic gene?

I am working on several unrelated enzymes that produce toxic products. All genes have been cloned into T7 promoter containing expression vectors, yet when transforming to non-expression hosts such...

18 September 2017 7,924 7 View