For ascorbate determinations, 0.5 g cells were packed in 2 mL tubes, resuspended in 1.5 mL of a 5% w/v metaphosphoric acid solution and frozen in liquid nitrogen.
please see this article: Liu X, Fan L, Yin S, Chen H, Hu H (2019) Molecular mechanisms of fumonisin B1-induced toxicities and its applications in the mechanism-based interventions. Toxicon, 167: 1-5.