I am isolating NK cells from PBMNC. The yield of NK cells at the end of culture was found to be less than 60%.

Kindly recommend me

(1) The NK culture media that can activate and enhance expansion of NK cells

(2) Do I need to boost my culture with IL-2 during every monitoring?

(3) What is the concentration of IL-2 to use?

(4) Is IL-2 available commercially?

(5) Any idea of using Optimiser plus IL-2 and other media?

(6) Any suggestions to increase the purity of NK cells

Cheers