I am trying to add 3 fragments to a vector. All 3 fragments are different sizes (125 bp, 4578 bp, 1445 bp). But I can't reach the correctly combined plasmid. I use 1 uL of each fragment and backbone, and I add the total (4uL) of gibson reaction (2x concentration). I tried chemical transformation and electroporation to send my gibson reaction to the cell and I can't observe any colonies. What am I doing wrong, what should I do?