Hi, I ran the digested plasmid on gel and purified it separately with NEB and QIAgen gel extraction kits, but the results came like this, how should I interpret these results? Where am I going wrong or are these results normal for digested plasmid?
This is not uncommon when attempting to quantify DNA after agarose gel purification. Hence I quit doing in decades ago. Instead, take an aliquot of your purified digested plasmid and run it in a gel (with EtBr) along with a DNA ladder with known quantities for the bands (i.e. NEB markers). You can compare the intensity of your digested plasmid band to the ladder bands and get a pretty close approximation of the concentration of your DNA.