Beatriz María Fernández Santos

4 Questions 6 Answers 0 Followers

Questions related from Beatriz María Fernández Santos

What should be done differently in a second round of ligation?

Hi, everyone I am using a 4.3kb vector to clone both a promotor and an enhancer. I already have a positive miniprep with no mutations in the promotor, therefore I took some of this plasmid,...

08 August 2019 8,790 3 View

How to avoid losing so much DNA after purification?

Good morning, everyone I am amplyfing and then digesting a specific insert that I later want to clone into a vector. OK. The PCR from genome works great and I obtain concentrations of 400-500...

06 June 2019 2,404 8 View

Why am I not getting my right size band PCR?

Hello, everyone I have been struggling to amplify a mouse promotor for almost 2 months now. This fragment is: - Roughly 1500-1700 pb depending on the set of primers - GC content of about 60%,...

12 December 2018 7,313 3 View

No PCR bands at all?

Hello, everyone I've been struggling for almost a month already when amplifying a promoter from the whole mouse genome: - I already designed 4 primers and have been trying them all (meaning a...

11 November 2018 6,957 4 View