Adenovirus titration

More Ashmenta Robrams's questions See All

Can I generate Dendritic cells from NSG mice?

Hi I'm trying to generate murine DCs from NSG HLA A2 mice ,,, using 20ng GM-CSF However, all what I get is MHCII neg, F4/80 hi, which show they are macrophages ,,, So I wonder can I generate...

26 March 2019 1,186 3 View

Can I differentiate between mouse M1 and M2 Macrophages in FACs using these Abs only?

Hi every one I wonder if I can differentiate between M1 and M2 macrophages in mouse tumours by using the following markers only? CD11b, CD11c, Ly6C, Ly6G, F4/80, MHCII If not ,,,which Ab I...

20 June 2018 7,317 2 View

How much time dendritic cells need to process adeno virus to be presented on their surface?

Hi I need to infect dendritic cells derived from mouse bone marrow with adenovirus then co culture them with T cells I'm wondering how much time the dendritic cells need to process the virus...

05 May 2018 9,645 2 View

What are the best markers for immature Dendritic cells derived from mouse bone marrow?

Hi I need a good panel for my FACS experiment which I 'll differentiate (mature DCs- which pulsed with peptide) and immature DCs ? Thanks

04 May 2018 2,145 3 View

Please help I need a good protocol for adenovirus titration???

Hi everyone I'm trying to titre some stocks of adenovirus that I isolated from infected 293a and I need a protocol to see the infection efficiency as the plaque forming assay However, I tried...

24 February 2018 7,073 3 View

How many nM that I give to my mice according to the following info?

Hi I give my mice 15mg/kg of a specific drug So how many nanomolar I give to my mice If the drug's formula weight is 439.52g/Mol ?? Thanks

27 January 2018 5,819 3 View

Pulsing mouse splenocytes with virus

Hello everyone I wonder how can I pulse my mouse splenocytes with virus to be presented on MHC for co-culture experiments how many particles is enough for pulsing ,,, 10 virus / 1 cell? 50virus...

25 July 2017 4,223 2 View

What the shape of cancer cells mean invivo?

Hi What does the different shape of cells mean, invivo subcuatneous, cells are from single sorting spindle shape and round shape of pancreatic cancer cell line from KPC mouse ? thanks

20 May 2017 3,003 1 View

Monocytic vs Granulocytic myeloid-derived suppressor cells (MDSCs)?

Is there any difference when I find Monocytic or Granulocytic myeloid-derived suppressor cells ( MDSCs ) in the tumours? Thanks

17 April 2017 2,175 1 View

What is the X ray irradiation dose to stop proliferation of cancer cell lines?

I need to test the proliferation ability of anti-Her2 CAR T cells in response to Her2 expressing cell lines using 3H incorporation assay ,,, First I need to stop proliferation of my cancer cells...

31 March 2017 7,268 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How to make Boehm titration ?

Hello Everyone, While doing Boehm titration, We use nahco3 in order to determine carboxylic acids. Then the mixture was agitated. After a while, the solution was filtred and HCl was added. After a...

29 July 2024 4,048 4 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View