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Questions related from Asha Rao
How can we do western blot of caspase 3 antibody for the cytoplasmic fraction ? Please share the protocol. Also i am isolating the mitochondrial pellet. so how do i determine that which part is...
last month 6,620 3 View
Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...
19 July 2024 2,511 0 View
Kindly confirm can we do MTT assay for quantification of apoptosis. Is MTT Assay a valid assay to quantify apoptotic cells ? What about LDH assay ?..how is it better for apoptosis quantification...
09 July 2024 5,545 2 View
I am determining the mitochondrial membrane potential of mitochondria (isolated from rat brain tissue) using Rhodamine 123 dye. In my experimental procedure, i take the reading of...
09 May 2024 5,119 1 View
Kindly confirm: Can we take PTZ (Pentylenetetrazole) model as model of Status Epilepticus and clinically co-relate with human MTLE its findings ? If we want to study acute changes of...
09 January 2024 7,695 3 View
I am working on animal model of Epilepsy. I wanted to ask if it is ethically allowed to perform neurobehavioural tests (T-maze, rota-rod etc) on my acute model rats (maximum survival days: 72...
02 January 2024 3,333 0 View
Being an activator of Mitochondrial calcium uniporter, how can spermine (polyamine) decrease calcium concentration in mitochondria ? In my experiments, spermine is decreasing calcium concentration...
20 June 2023 4,764 0 View
Kindly confirm how can we interpret data of delta Ct in RT-PCR Results? We have just control tissue, not any test sample; thereby delta delta Ct values can't be find out. We hv just delta Ct...
05 December 2022 3,097 2 View
I have mitochondrial pellet with me kept in homogenization buffer. Now i need to perform BCA. Kindly confirm how much quantity of RIPA Lysis buffer and protease inhibitor is to be added to that...
30 June 2022 8,325 0 View
I performed X-Rhod 1 staining for determining calcium concentration in rat brain mitochondrial fractions. But, after calculations, in some samples the concentration is coming negative, which is...
09 May 2022 1,743 0 View
Is it possible to form paraffin embedded tissue sections from tissues stored at -80*C ? Can we take that snap frozen tissues and start processing (formalin-2 days, then xylene, ethanol steps...:...
09 May 2022 8,055 3 View
For determination of ROS in mitochondrial fraction using DCFDA, How to quantify the concentration of isolated mitochondrial fraction? using nanospectrometer or some other technique ? If i perform...
05 May 2022 4,715 2 View
I have isolated mitochondrial pellet from rat brain, now how i can determine its calcium concentration using X-Rhod 1 ? On surfing from net, the protocols are coming from cells. But i have...
24 March 2022 1,498 1 View
Does any human cell line can replicate blood brain barrier microenvironment to be used for study of epilepsy and related pathologies? Kindly confirm
24 February 2022 6,228 4 View
Kindly provide 1-2 papers showing evidance about the mortality rate in hamster model for pancreatic cancer development (using BOP).
11 October 2021 9,174 6 View
For isolation of mitochondria from rat hippocampus, chilled isolation medium (0.25 M sucrose containing 10 mM Tris–HCL, pH 7.4, 1 mM EDTA, and BSA 250 lg/mL) is made. Can we make this solution...
08 October 2021 5,258 7 View
