15 Questions 11 Answers 0 Followers
Questions related from Asha Rao
Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...
19 July 2024 2,370 0 View
Kindly confirm can we do MTT assay for quantification of apoptosis. Is MTT Assay a valid assay to quantify apoptotic cells ? What about LDH assay ?..how is it better for apoptosis quantification...
09 July 2024 5,453 2 View
I am determining the mitochondrial membrane potential of mitochondria (isolated from rat brain tissue) using Rhodamine 123 dye. In my experimental procedure, i take the reading of...
09 May 2024 5,029 1 View
Kindly confirm: Can we take PTZ (Pentylenetetrazole) model as model of Status Epilepticus and clinically co-relate with human MTLE its findings ? If we want to study acute changes of...
09 January 2024 7,635 3 View
I am working on animal model of Epilepsy. I wanted to ask if it is ethically allowed to perform neurobehavioural tests (T-maze, rota-rod etc) on my acute model rats (maximum survival days: 72...
02 January 2024 3,286 0 View
Being an activator of Mitochondrial calcium uniporter, how can spermine (polyamine) decrease calcium concentration in mitochondria ? In my experiments, spermine is decreasing calcium concentration...
20 June 2023 4,708 0 View
Kindly confirm how can we interpret data of delta Ct in RT-PCR Results? We have just control tissue, not any test sample; thereby delta delta Ct values can't be find out. We hv just delta Ct...
05 December 2022 3,020 2 View
I have mitochondrial pellet with me kept in homogenization buffer. Now i need to perform BCA. Kindly confirm how much quantity of RIPA Lysis buffer and protease inhibitor is to be added to that...
30 June 2022 8,292 0 View
I performed X-Rhod 1 staining for determining calcium concentration in rat brain mitochondrial fractions. But, after calculations, in some samples the concentration is coming negative, which is...
09 May 2022 1,691 0 View
Is it possible to form paraffin embedded tissue sections from tissues stored at -80*C ? Can we take that snap frozen tissues and start processing (formalin-2 days, then xylene, ethanol steps...:...
09 May 2022 8,009 3 View
For determination of ROS in mitochondrial fraction using DCFDA, How to quantify the concentration of isolated mitochondrial fraction? using nanospectrometer or some other technique ? If i perform...
05 May 2022 4,668 2 View
I have isolated mitochondrial pellet from rat brain, now how i can determine its calcium concentration using X-Rhod 1 ? On surfing from net, the protocols are coming from cells. But i have...
24 March 2022 1,466 1 View
Does any human cell line can replicate blood brain barrier microenvironment to be used for study of epilepsy and related pathologies? Kindly confirm
24 February 2022 6,183 4 View
Kindly provide 1-2 papers showing evidance about the mortality rate in hamster model for pancreatic cancer development (using BOP).
11 October 2021 9,130 6 View
For isolation of mitochondria from rat hippocampus, chilled isolation medium (0.25 M sucrose containing 10 mM Tris–HCL, pH 7.4, 1 mM EDTA, and BSA 250 lg/mL) is made. Can we make this solution...
08 October 2021 5,219 7 View
