Is there any specific strain of algae available for producing bioethanol or biodiesel alone? What is the percentage composition of biomass and/or lipids in a typical alga? Please clarify this.
1. The products of Bioethanol or biodiesel is depended strains what kind of products much produced. If the carbohydrate group much produced the strain, it is a bioethanol producing available stain. If the a strain produce much lipid, it may can be used as biodiesel producing strain.
2. Some high lipid producing stain, such as Botryococcus may have oil as high as 50-70% of its dry weight .
May this can help you~
The chemical compositon varies a lot between different species. Some species predominately store carbon as carbohydrates (will be suitable for ethanol production), other use lipid storage. More important are the growth conditions: the biochemical composition of algae varies a lot with light and nutrient availability, temperature, and growth stage. In certain species N limitation results in a significant increase in lipid content. In most species fast growing cells will not produces to much storage compounds (e.g. their lipid content will be under 20%).
Here some further info:
http://www.fao.org/docrep/003/w3732E/w3732e07.htm
http://cdn.intechweb.org/pdfs/17890.pdf
Thank you for your answers. But how to identify if a particular alga is predominantly having lipid or carbohydrate? Is there any separate procedure available for algae? or we need to follow general biochemical assays for carbohydrate and lipid.
The following information may be useful for your research
Fractionation & Extraction fractionation technologies (for converision of algal biomass into carbohydrates, proteins, and lipids) to be improved in terms of cost, energy‐use, and scalability. Yield of desired product is also important
Ref: The Promise and Challenge of Algae as Renewable Sources of Biofuels
Biomass Program Webinar; September 8, 2010
www1.eere.energy.gov/biomass/pdfs/algae_webinar.pdf
Thank you all for the answers. It was helpful. Have a nice day.
You may find answers to your questions in the following papers. Most of them is possible to find on line in my ResearchGate page
Vilem Zachleder
DOUSKOVA, I.; DOUCHA, J.; LIVANSKY, K.; MACHAT, J.; NOVAK, P.; UMYSOVA, D.; ZACHLEDER, V.; VITOVA, M.: Simultaneous flue gas bioremediation and reduction of microalgal biomass production costs. Appl Microbiol Biotechnol 232: 183-191, 2009.
BRÁNYIKOVÁ, I.; MARŠÁLKOVÁ, B.; DOUCHA, J.; BRÁNYIK, T.; BIŠOVÁ, K.;ZACHLEDER, V.; VÍTOVÁ, V.: Microalgae - novel highly-efficient starch producers. Biotechnol Bioeng 108: 766-776. 2011.
PŘIBYL, P.; CEPÁK, V.; ZACHLEDER, V.: Production of lipids in 10 strains of Chlorella and Parachlorella, and enhanced lipid productivity in Chlorella vulgaris. Appl Microbiol Biotechnol 94: 549–561, 2012
LI, X.; PŘIBYL, P.; BIŠOVÁ, K.; KAWANO, S.; CEPÁK, V.; ZACHLEDER, V.; ČÍŽKOVÁ, M.; BRÁNYIKOVÁ, I.; VÍTOVÁ, M.: The Microalga Parachlorella kessleri – a novel highly efficient Lipid producer. Biotechnol Bioeng 110: 97–107, 2013.
MIZUNO, Y.; SATO, A.; WATANABE, K.; HIRATA, A.; TAKESHITA, T.; OTA, S.; SATO, N.; ZACHLEDER, V.; TSUZUKI, M.; KAWANO, S.: Sequential accumulation of starch and lipid induced by sulfur deficiency in Chlorella and Parachlorella species. Bioresour Technol 129: 150–155, 2013.
PŘIBYL, P.; CEPÁK, V.; ZACHLEDER, V.: Production of lipids and formation and mobilization of lipids bodies on Chlorella vulgaris. J Appl Phycol 25: 545–553, 2013.
FERNANDES, B.; TEIXEIRA, J.; DRAGONE, G.; VICENTE, A.A.; KAWANO, S.; BIŠOVÁ, K.; PŘIBYL, P.; ZACHLEDER, V. ; VÍTOVÁ, M.: Relationship between starch and lipid accumulation induced by nutrient depletion and replenishment in the microalga Parachlorella kessleri. Bioresour Technol 144: 268-274, 2013.
Simply the % of microalgal oil can be calculated using this formula
Oil (%) = weight of extracted oil(g)/weight of microalgal biomass x 100
and the carbohydrate content can be estimated using DNS method or Phenol sulfuric acid method. Another method i.e Nile red staining method is also used for quantification of intracellular oil from microalgae
Habe a look at Table 1 int his paper:
www.biotechnologyforbiofuels.com/content/6/1/150
For bio-ethanol production you need green algae ( Ulva spp. ) but not only them. These species have a vast distribution around the world, easy to grow and containing a lot of available carbohydrates for ethanol production (40-68 %). The methods for saccharification are DNS and Phenol sulfuric acid. For further info you can contact with me anytime.
There are some information on different carbohydrate, lipid and ptn content of different sp. of microalgae are available at following review paper.
Third generation biofuels from microalgae, Authors: Giuliano Dragone, Bruno Fernandes, António A. Vicente, and José A. Teixeira
Journal name: Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology A. Méndez-Vilas (Ed)
This is open access journal so You can download it from google
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