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Questions related from Anita Ma
This is my PCR 1.2% agarose gel result. I used a 25uL reaction system (0.3uM primers, 12.5uL Taq master mix, 1uL genome template and 5.5uL H2O). The primer Tm is 62℃, and reaction procedure is 94℃...
15 August 2023 1,360 5 View
I want to find out potential mechanisms of the effects of a certain gene on recombinant protein productivity . So proteomics analysis was performed between a high expression of my testing gene and...
28 March 2022 2,894 2 View
I am doing over expression of an endogenous target gene in a CHO cell line. When I did PCR (whole length of the gene) to verify the existence of my target gene, I could only see the gel band when...
28 October 2021 6,427 6 View
After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...
26 February 2021 5,947 3 View
