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Questions related from Anara Alshanbayeva
Hi everyone, Does anyone have a protocol for IP of HDL from mouse plasma? But the plasma was prior to IP diluted with optiprep for density gradient. The goal is in the end to sequence HDL-RNAs and...
31 July 2019 7,284 1 View
Does anyone have experience in separating lipoproteins from proteins in mouse plasma with a simple precipitation or any other similar method (preferably, not expensive), so that the RNA bound to...
31 July 2019 7,753 0 View
Does anyone have experience isolating protein-bound RNAs from circulation using a density gradient? (Optiprep, sucrose, etc.) What's the chance of interaction between the protein & the RNA...
01 March 2019 3,679 0 View
Even mouse plasma that has (by observing visually) absorbance higher than 0.3 at A414nm. According to publications, this is a high level of hemolysis in humans. Does the same cutting point (0.3)...
06 July 2018 2,447 0 View
hi everyone, i am having interesting observations on reflowing AZ 40XT, the reflow suggested by the manufacturer (120C for 30 seconds) does not give a desired round surface profile. I have tried...
18 July 2017 8,006 6 View
Dear all, I am in need to intensively learn how to draw and design microfluidic chip (control and flow layers separately) using AutoCAD. I would like to ask the experts in this area for any...
26 February 2017 8,905 7 View
I have had this issue twice already. I have a gene of interest in a pGBT9 plasmid, I made restriction enzyme digestion (using two enzymes: EcoRI and BlpI). I treated with BlpI for 1 hour, added...
16 June 2014 1,748 6 View
