Anara Alshanbayeva

11 Questions 9 Answers 0 Followers

Questions related from Anara Alshanbayeva

Mouse plasma HDL immunoprecipitation protocol (for subsequent sequencing of HDL-bound RNA)?

Hi everyone, Does anyone have a protocol for IP of HDL from mouse plasma? But the plasma was prior to IP diluted with optiprep for density gradient. The goal is in the end to sequence HDL-RNAs and...

31 July 2019 7,314 1 View

Lipoprotein isolation/precipitation from proteins in mouse plasma?

Does anyone have experience in separating lipoproteins from proteins in mouse plasma with a simple precipitation or any other similar method (preferably, not expensive), so that the RNA bound to...

31 July 2019 7,782 0 View

Separation of Argonaute proteins from HDL in plasma?

Has anyone worked on separation of Argonaute proteins from HDL in plasma, so that RNA binding is not disturbed (with the final goal of sequencing RNAs bound to each)? A simple method would be...

31 July 2019 9,694 1 View

Isolating protein-bound RNAs with a density gradient ?

Does anyone have experience isolating protein-bound RNAs from circulation using a density gradient? (Optiprep, sucrose, etc.) What's the chance of interaction between the protein & the RNA...

01 March 2019 3,711 0 View

Are assessment of hemolysis by absorbance at A414nm or calculations by M.Harboe method necessary?

Even mouse plasma that has (by observing visually) absorbance higher than 0.3 at A414nm. According to publications, this is a high level of hemolysis in humans. Does the same cutting point (0.3)...

06 July 2018 2,476 0 View

Is there a way to take into account the effect of hemolysis level to mouse plasma total RNA sequencing?

Several publications indicate hemolysis levels alter differential expression of a number of miRNAs in human plasma, in some cases up to 40-88% of miRNAs (does anyone have reference to such mouse...

06 July 2018 9,805 0 View

Reflow of AZ40 XT photoresist for multilayer soft lithography generated bubbles

hi everyone, i am having interesting observations on reflowing AZ 40XT, the reflow suggested by the manufacturer (120C for 30 seconds) does not give a desired round surface profile. I have tried...

18 July 2017 8,035 6 View

Cleaning microfluidic chip from punching holes in two-layer microfluidic device?

Hello everyone, I am experiencing a problem with double layer microfluidic fabrication. I am going to eventually use this device on human cells, thus the environment needs to be appropriate. I...

17 March 2017 5,454 3 View

Microfluidic chip design using AutoCAD?

Dear all, I am in need to intensively learn how to draw and design microfluidic chip (control and flow layers separately) using AutoCAD. I would like to ask the experts in this area for any...

26 February 2017 8,932 7 View

Algorithm for automated flow cytometry analysis using R language?

Hi, everyone. I'm working on creating a pipeline for automated flow cytometry analysis using R language. I have been mostly used a package called openCyto; however, used several plugin functions...

11 July 2016 1,082 6 View

Why is my ligation or transformation not working?

I have had this issue twice already. I have a gene of interest in a pGBT9 plasmid, I made restriction enzyme digestion (using two enzymes: EcoRI and BlpI). I treated with BlpI for 1 hour, added...

16 June 2014 1,775 6 View