What are the most important explanatory assets ?

06 December 2020 5,474 2 View

What are the methods of writing the interpretation ?

06 December 2020 3,267 3 View

Cell culture techniques: how to deactivate a viral infection while preserving cytokine storm markers?

Hello, I am trying to measure the cytokine storm markers following SARS-Cov-2 viral infection of lung cells and macrophages under different conditions. However, finding a safe way to deactivate...

05 November 2020 1,174 4 View

How can I calculate the am ount required to prepare the following buffer?

ammonium sulfate 0.17 M - polyethylene glycol 4000 25.5% (w/v) - glycerol 15% solution How can I calculate the amount required to prepare the following buffer? I want to prepare 1000 microliter

03 October 2020 4,018 5 View

Why some viral proteins are unable to express and purify in pET28a expression and purification system?

Dear All, I am trying to clone and purify codon-optimized viral proteins using pET28a expression system. Even though I have the perfect insert in the recombinant plasmid, there was absolutely no...

24 September 2020 3,635 8 View

Non fluorescent E. coli GFP?

I Have a problem with E. coli GFP, I am culturing them in LB medium/ampicillin + IPTG, but not all the cells fluorescent after UV excitation (fluorescence microscope) although they grew in the...

15 September 2020 4,636 1 View

How are parents guiding their children with learning difficulties?

Parents' educational level is essential in raising the level of education

14 September 2020 6,306 4 View

Is it possible to integrate ratio measures with ordinal survey scale?

24 June 2020 205 1 View

X-ray Experiments for antibody S309 Zero ini on COVID-19 ?

18 May 2020 3,666 6 View

Why encrypt Bloom filter and query in a different way in the following paper in searchable encryption?

I was read in paper in searchable encryption that encrypt bloom filter index in the following way: 1- generate secret key to encrypt bloom filter and query is: the secret key sk (M1, M2, s ),...

03 March 2020 1,448 5 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Which alginate is best for making gel for wound dressing application?

We are looking into utilizing alginate as a gel coating on an HDPE substrate for drug delivery. I would like to ask the community about their experience with alginate and which commercial sources...

15 February 2021 4,319 4 View