20 Questions 64 Answers 0 Followers
Questions related from Adam L Vanwert
I have found very few examples of successful gene delivery to the small intestine in the literature. Is anyone here working on such a project? I believe it has a huge potential for correcting...
21 March 2021 2,434 4 View
I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...
21 February 2021 6,245 4 View
We are performing an eosin Y displacement assay wherein eosin Y is quenched by a copper(II) based macrocycle, then released by organic anions. The release causes recovery of fluorescence. If we...
12 June 2020 1,660 0 View
Based on the solubility of copper (II) oxalate it should be supersaturated at any oxalate concentration in the uM range, however when I add 1 mM copper nitrate and more I don't see a precipitate...
26 January 2020 1,943 1 View
I am performing a derivatization in serum. I precipitate protein with HCl (10% concentrated HCl final) , pellet the protein, collect supernatant, and react the supernatant in the HCl at 90...
13 November 2019 6,362 1 View
PITC reacts with ammonia, primary, and secondary amines. Therefore, it should react with uric acid, correct?
26 June 2019 905 0 View
Hello, I have a question about PDAM that I hope someone can help me with. I would like to measure oxalate (a dicarboxylic acid) in aqueous samples, and I need to find a suitable fluorescent...
15 January 2019 9,857 3 View
What specific part of the ovary are CHO cells from? I can't find this information in the literature.
18 August 2018 8,838 4 View
I have been working with Chinese Hamster Ovary (CHO) cells for 13 or so years, and I have learned a lot of useful lessons about their stable transfection, maintenance of expression, and other...
19 December 2017 3,890 1 View
I would like to purchase highly crosslinked polystyrene sulfonate. I mean 80 or more% crosslinked. Would I have to synthesize it instead?
04 July 2017 2,554 5 View
Calcium carbonate and calcium oxalate are both highly insoluble in water. Is it possible to shift the calcium from oxalate to carbonate if enough carbonate is supplied (in vitro).
14 May 2017 3,353 2 View
I've been looking at various protease inhibitor cocktails and it's a bit frustrating that when I'm lysing CHO cells I need such a small volume of protease inhibitor cocktail that I would need a...
16 April 2017 9,961 6 View
I've performed a reaction several times over the past few months, and this is the first time I experienced a new problem. Normally I add diethylenetriamine to acetonitrile to serve as a...
14 October 2016 4,319 4 View
I'm really struggling with a reaction. I'm trying to react these compounds in acetonitrile to form a cyclic structure with two of each molecule. I add the terephthalaldehyde drop wise in...
06 May 2016 7,370 11 View
Please share if you know any immunologists/ scientists in the field. If the mRNA based vaccines get into the fetal blood in a pregnant patient, what are the chances of negative selection, in...
01 January 1970 10,076 2 View
As a pharmacologist and pharmacist I have been thinking about these mRNA vaccines quite a lot. In a previous discussion I asked if the mRNA sequence of the vaccines has been published. So far...
01 January 1970 7,318 2 View
From the articles I've read on COVID-19 and ACE2 (ACE2 being the binding target for the virus), it appears that both inhaled ACE2 and systemic ACE2, in a recombinant form, may be the solution to...
01 January 1970 5,222 18 View
https://youtu.be/WbNDvUHwOjc?si=Nn0jlBIZh2hcEXEu Finished my video on the octanol-water partition coefficient! Took me quite a while to find a dye that would distribute into the octanol when acid...
01 January 1970 1,870 0 View
Please see my image. I have read that aminoglycoside antibiotics disrupt the lipopolysaccharide barrier in bacteria by replacing calcium and magnesium with their amines. Normally calcium and...
01 January 1970 966 0 View
For all my fellow scientists and other researchers, I designed a new web app for connecting researchers who have hard-earned data, but not enough results for a full "story". The main goal of the...
01 January 1970 7,827 0 View
